Baggish M S, Poiesz B J, Joret D, Williamson P, Refai A
Health Science Center, SUNY, Syracuse.
Lasers Surg Med. 1991;11(3):197-203. doi: 10.1002/lsm.1900110302.
Concentrated tissue culture pellets infected with human immunodeficiency virus (HIV) containing 1 x 10(7) cells/ml were vaporized by means of a carbon dioxide laser. The vaporous debris resulting from the laser's impact were evacuated through sterile silastic tubing, then bubbled through sterile culture medium (RPMI) positioned in series with a commercial smoke evacuator. No HIV DNA was detected in the culture medium flask. Tissue culture studies of the silastic collection tubing revealed p24 HIV gag antigen in 3 of 12 tube segments at the end of 1 week and in 1 of 12 tube segments at 2 weeks. No sustained infection of HIV cultured cells was observed at the 28th day. Polymerase chain reaction (PCR) analysis of particulate debris obtained from the silastic collection tubing was positive from proviral HIV DNA in both immediately sampled and day 14 cultured material.
含有1×10⁷个细胞/毫升的感染了人类免疫缺陷病毒（HIV）的浓缩组织培养沉淀物，通过二氧化碳激光进行汽化。激光冲击产生的气态碎片通过无菌硅橡胶管排出，然后通过与商用烟雾抽吸器串联放置的无菌培养基（RPMI）鼓泡。在培养基烧瓶中未检测到HIV DNA。对硅橡胶收集管进行的组织培养研究显示，在1周结束时，12个管段中有3个检测到p24 HIV gag抗原，在2周时，12个管段中有1个检测到。在第28天未观察到HIV培养细胞的持续感染。对从硅橡胶收集管获得的颗粒碎片进行的聚合酶链反应（PCR）分析显示，在立即采样的和第14天培养的材料中，前病毒HIV DNA均呈阳性。
