Meng Xiang-Ren, Guo Jun, Zhao Qian-Jun, Ma Yue-Hui, Guan Wei-Jun, Liu Di, Di Ran, Qiao Hai-Yun, Na Ri-Su
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Yi Chuan. 2008 Dec;30(12):1585-90. doi: 10.3724/sp.j.1005.2008.01585.
PCR-RFLP was applied to analyze the polymorphism of MSTN gene UTR in 345 sheep that comprised of eleven sheep breeds, namely Texel sheep, Charolais sheep, Small-tailed Han sheep, Monggolian sheep, Ujumqin sheep, Altay Fat-rumped sheep, Hulunbeir sheep, Tashikurgan sheep, Duolang sheep, Hu sheep, and Gangba sheep. A 271 bp and a 1 003 bp long PCR products were digested with Mboand Bsato demonstrate polymorphism in the eleven sheep breeds, which were all at Hardy-Weinberg equilibrium (P>0.05). The distribution of 3 genotypes in 11 sheep breeds was significantly different (P<0.01). Digestion of the PCR products with HpyCH4 proved that 9 domestic local sheep breeds were different from Texel sheep in the SNP site that was associated with muscularity. The individual mutation base could generate the motifs for miRNA in the 3'UTR, and sequencing analysis demonstrated high frequency of mutation in the 3'UTR region.
采用聚合酶链反应-限制性片段长度多态性方法（PCR-RFLP），对包括特克塞尔羊、夏洛莱羊、小尾寒羊、蒙古羊、乌珠穆沁羊、阿勒泰大尾羊、呼伦贝尔羊、塔什库尔干羊、多浪羊、湖羊和岗巴羊11个绵羊品种的345只绵羊的肌肉生长抑制素（MSTN）基因非翻译区（UTR）多态性进行分析。用MboⅠ和BsaⅠ对长度分别为271 bp和1 003 bp的PCR产物进行酶切，以检测11个绵羊品种中的多态性，所有品种均处于哈迪-温伯格平衡状态（P>0.05）。11个绵羊品种中3种基因型的分布存在显著差异（P<0.01）。用HpyCH4Ⅱ对PCR产物进行酶切证明，9个国内地方绵羊品种在与肌肉发育相关的单核苷酸多态性（SNP）位点上与特克塞尔羊不同。单个突变碱基可在3'UTR中产生微小RNA（miRNA）的基序，测序分析表明3'UTR区域的突变频率较高。
