Pan Yuan, Li Siyi, Zhang Qiu, Li Jiaqi, Song Chenyu, Kong Lingchao, Liu Yining, Hou Sibing, Li Shuaitong, Liu Qingkun, Xia Decui, Wang Zeying
College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
J Genet Eng Biotechnol. 2024 Jun;22(2):100372. doi: 10.1016/j.jgeb.2024.100372. Epub 2024 Mar 27.
The myostatin (MSTN) gene exhibits significant nucleotide sequence variations in sheep, impacting growth characteristics and muscular traits of the body. However, its influence on specific growth traits in some sheep remains to be further elucidated. This study utilized single nucleotide polymorphism sequence analysis to investigate the role of the MSTN gene in meat production performance across four sheep breeds: Charolais sheep, Australian White sheep, crossbreeds of Australian White and Small-tailed Han, and crossbreeds of Charolais and Small-tailed Han. At a SNP locus of the MSTN gene, the C2361T site was identified, with three genotypes detected: CC, CT, and TT, among which CC predominated. Gene substitution effect analysis revealed that replacing C with T could elevate the phenotypic value. Comparative analysis of data from different genotypes within the same breed highlighted the superiority of CC and TT genotypes in phenotypic values, underscoring the significance of specific genotypes in influencing key traits. Contrasting the performance of different genotypes across breeds, Charolais sheep and Charolais Han hybrids demonstrated superiority across multiple indicators, offering valuable insights for breeding new sheep varieties. Analysis of gender effects on growth characteristics indicated that ewes exhibited significantly wider chest, waist, and hip widths compared to rams, while rams displayed better skeletal growth and muscle development. Additionally, the MSTN gene also exerted certain effects on lamb growth characteristics, with the CC genotype closely associated with weight. These findings not only contribute crucial insights for sheep breeding but also pave the way for future research exploring the interaction of this gene with others.
肌肉生长抑制素（MSTN）基因在绵羊中表现出显著的核苷酸序列变异，影响着绵羊的生长特性和身体肌肉性状。然而，其对某些绵羊特定生长性状的影响仍有待进一步阐明。本研究利用单核苷酸多态性序列分析，探究MSTN基因在夏洛莱羊、澳洲白羊、澳洲白羊与小尾寒羊杂交后代以及夏洛莱羊与小尾寒羊杂交后代这四个绵羊品种肉用生产性能中的作用。在MSTN基因的一个单核苷酸多态性位点，鉴定出C2361T位点，检测到三种基因型：CC、CT和TT，其中CC基因型占主导。基因替代效应分析表明，用T替代C可提高表型值。同一品种内不同基因型数据的比较分析突出了CC和TT基因型在表型值方面的优势，强调了特定基因型对关键性状的影响。对比不同品种间不同基因型的表现，夏洛莱羊和夏洛莱羊与小尾寒羊杂交后代在多个指标上表现出优势，为培育新的绵羊品种提供了有价值的见解。性别对生长特性影响的分析表明，母羊的胸围、腰围和臀围比公羊显著更宽，而公羊的骨骼生长和肌肉发育更好。此外，MSTN基因对羔羊生长特性也有一定影响，CC基因型与体重密切相关。这些发现不仅为绵羊育种提供了关键见解，也为未来探索该基因与其他基因相互作用的研究铺平了道路。
