小鼠成纤维细胞中细胞色素c的缺失会破坏呼吸复合体I和IV的组装/稳定性。
Lack of cytochrome c in mouse fibroblasts disrupts assembly/stability of respiratory complexes I and IV.
作者信息
Vempati Uma D, Han Xianlin, Moraes Carlos T
机构信息
Department of Neurology and Department of Cell Biology and Anatomy, University of Miami Miller School of Medicine, Miami, Florida 33136, USA.
出版信息
J Biol Chem. 2009 Feb 13;284(7):4383-91. doi: 10.1074/jbc.M805972200. Epub 2008 Dec 15.
Abstract
Cytochrome c (cyt c) is a heme-containing protein that participates in electron transport in the respiratory chain and as a signaling molecule in the apoptotic cascade. Here we addressed the effect of removing mammalian cyt c on the integrity of the respiratory complexes in mammalian cells. Mitochondria from cyt c knockout mouse cells lacked fully assembled complexes I and IV and had reduced levels of complex III. A redox-deficient mutant of cyt c was unable to rescue the levels of complexes I and IV. We found that cyt c is associated with both complex IV and respiratory supercomplexes, providing a potential mechanism for the requirement for cyt c in the assembly/stability of complex IV.
摘要
细胞色素c（cyt c）是一种含血红素的蛋白质，它参与呼吸链中的电子传递，并作为凋亡级联反应中的信号分子。在此，我们研究了去除哺乳动物细胞色素c对哺乳动物细胞呼吸复合体完整性的影响。细胞色素c基因敲除小鼠细胞的线粒体缺乏完全组装好的复合体I和IV，且复合体III的水平降低。细胞色素c的氧化还原缺陷型突变体无法挽救复合体I和IV的水平。我们发现细胞色素c与复合体IV和呼吸超级复合体都有关联，这为细胞色素c在复合体IV组装/稳定性中的需求提供了一种潜在机制。
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