Kamei Yasuhiro, Suzuki Motoshi, Watanabe Kenjiro, Fujimori Kazuhiro, Kawasaki Takashi, Deguchi Tomonori, Yoneda Yoshihiro, Todo Takeshi, Takagi Shin, Funatsu Takashi, Yuba Shunsuke
Radiation Biology Center, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto, Japan.
Nat Methods. 2009 Jan;6(1):79-81. doi: 10.1038/nmeth.1278. Epub 2008 Dec 14.
We developed infrared laser-evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock-mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.
我们开发了红外激光诱发基因操纵器（IR-LEGO），这是一种经过优化的显微镜系统，可在不造成光化学损伤的情况下加热细胞。红外辐射以功率依赖的方式导致体外微环境产生可重复的温度变化。当应用于活体秀丽隐杆线虫时，与440纳米染料激光相比，IR-LEGO能以更高效且危害更小的方式诱导目标单细胞中热休克介导的转基因表达，并引发生理相关的表型反应。
