量子点作为用于农用化学品暴露生物标志物的多重免疫分析中的报告分子。
Quantum Dots as Reporters in Multiplexed Immunoassays for Biomarkers of Exposure to Agrochemicals.
作者信息
Nichkova M, Dosev D, Davies A E, Gee S J, Kennedy I M, Hammock B D
机构信息
University of California, Davis, California.
出版信息
Anal Lett. 2007 Jan;40(7):1423-1433. doi: 10.1080/00032710701327088.
Abstract
The application of quantum dots (QDs) as labels in immunoassay microarrays for the multiplex detection of 3-phenoxybenzoic acid (PBA) and atrazine-mercapturate (AM) has been demonstrated. PBA and AM are biomarkers of exposure to the pyrethroid insecticides and to the herbicide atrazine, respectively. Microarrays were fabricated by microcontact printing of the coating antigens in line patterns onto glass substrates. Competitive immunoassays were successfully performed using QDs (QD560 and QD620) as reporters. The multiplexed immunoassays were characterized by fluorescence microscopy and SEM. The application of QD fluorophores facilitates multiplex assays and therefore can contribute to enhanced throughput in biomonitoring.
摘要
量子点（QDs）作为标记物在免疫分析微阵列中用于多重检测3-苯氧基苯甲酸（PBA）和阿特拉津巯基尿酸（AM）的应用已得到证实。PBA和AM分别是接触拟除虫菊酯类杀虫剂和除草剂阿特拉津的生物标志物。通过将包被抗原以线状图案微接触印刷到玻璃基板上来制备微阵列。使用量子点（QD560和QD620）作为报告分子成功进行了竞争性免疫分析。通过荧光显微镜和扫描电子显微镜对多重免疫分析进行了表征。量子点荧光团的应用促进了多重分析，因此有助于提高生物监测的通量。
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