Cano A, Rivera J
Department of Obstetrics and Gynaecology, Universidad de Valencia, Spain.
Placenta. 1991 Mar-Apr;12(2):153-60. doi: 10.1016/0143-4004(91)90019-c.
A Mr29,000 serine phosphoprotein (P29) related to oestradiol receptor was studied in human term placenta with the use of a specific monoclonal antibody (D5). D5 was used with two different methods, immunohistochemistry and immunoradiometry. For immunohistochemistry, an indirect immunoperoxidase method was chosen to detect P29 in methacarn-fixed, wax-embedded sections. P29 was mostly confined to the syncytiotrophoblast surrounding placental villi, staining being positive in both cytoplasm and nuclei. The stroma of villi was negative. Content of P29 was uniformly high in crude placental cytosol, as measured by immunoradiometry assays. Specificity of D5 against P29 in placenta was tested by an immunoblotting technique. This showed a single band corresponding to a molecular weight of 29 kDa. The biological meaning of the finding is unknown at present.
利用一种特异性单克隆抗体（D5），对人足月胎盘内与雌二醇受体相关的一种29,000丝氨酸磷蛋白（P29）进行了研究。D5采用两种不同方法，即免疫组织化学和免疫放射测定法。对于免疫组织化学，选用间接免疫过氧化物酶法在甲醇 Carnoy 固定、石蜡包埋的切片中检测P29。P29主要局限于胎盘绒毛周围的合体滋养层，细胞质和细胞核染色均为阳性。绒毛间质为阴性。通过免疫放射测定法检测，粗制胎盘细胞溶质中P29含量始终很高。采用免疫印迹技术检测D5对胎盘中P29的特异性。结果显示一条对应分子量为29 kDa的单带。目前该发现的生物学意义尚不清楚。
