The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
The Key Laboratory of Mariculture, Ministry of Education, Fisheries College, Ocean University of China, Yushan Road 5, Qingdao 266003, PR China.
Mar Environ Res. 2014 Mar;94:7-15. doi: 10.1016/j.marenvres.2013.11.001. Epub 2013 Nov 12.
In this study, we isolated a CYP3A-like gene from ovary of the scallop (Chlamys farreri). High levels of CYP3A-like gene expression occur in the digestive gland and gonad, which suggested their role in the metabolism of steroids and xenobiotics. Scallops were exposed to a polycyclic aromatic hydrocarbons (PAHs), benzo[a]pyrene (B[a]P) for 10 days. The CYP4 and CYP3A-like gene can be up-regulated by B[a]P in a dose-dependent manner after 10 days exposure. But no induction of the CYP3A-like was observed in 10 μg/L B[a]P group. The CYP1A-like expression can only be induced by 0.025 μg/L B[a]P. 0.5 and 10 μg/L B[a]P caused significant DNA damage and 10 μg/L B[a]P can also lead to oxidative damage. These results demonstrate that the mollusk CYPs can be modulated by environmental pollutant, and the blocked induction of CYP3A-like and CYP1A-like expression probably results from the high genotoxicity and oxidative damage partly.
在这项研究中，我们从扇贝（海湾扇贝）的卵巢中分离出一个 CYP3A 样基因。CYP3A 样基因在消化腺和性腺中表达水平较高，这表明它们在类固醇和外源物质的代谢中发挥作用。扇贝暴露于多环芳烃（PAHs）、苯并[a]芘（B[a]P）中 10 天。在 10 天暴露后，CYP4 和 CYP3A 样基因可以被 B[a]P 以剂量依赖的方式上调。但是，在 10μg/L B[a]P 组中未观察到 CYP3A 样的诱导。CYP1A 样表达只能被 0.025μg/L B[a]P 诱导。0.5 和 10μg/L B[a]P 导致明显的 DNA 损伤，而 10μg/L B[a]P 也可导致氧化损伤。这些结果表明，软体动物的 CYP 可以被环境污染物调节，而 CYP3A 样和 CYP1A 样表达的阻断诱导可能部分归因于高遗传毒性和氧化损伤。
