Okamoto Akimitsu
Advanced Science Institute, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, 351-0198, Japan.
Org Biomol Chem. 2009 Jan 7;7(1):21-6. doi: 10.1039/b813595a. Epub 2008 Nov 6.
The development of a reaction for the detection of the presence/absence of one methyl group in a very long DNA strand is a chemically and biologically challenging research subject. Several newly designed chemical assays for the typing of DNA methylation are reported and discussed in this paper. A new concept of sequence-specific short-term methylation analysis, supported by a chemical basis, is the starting point for a novel methylation-typing assay, which will supersede conventional methods.
开发一种用于检测非常长的DNA链中一个甲基存在与否的反应，是一个具有化学和生物学挑战性的研究课题。本文报道并讨论了几种新设计的用于DNA甲基化分型的化学分析方法。一种基于化学基础的序列特异性短期甲基化分析新概念，是一种新型甲基化分型分析方法的起点，该方法将取代传统方法。
