Rowan Raymond, Moran Ciara, McCann Malachy, Kavanagh Kevin
Medical Mycology Unit, NICB, Department of Biology, National University of Ireland Maynooth, Co., Kildare, Ireland.
Biometals. 2009 Jun;22(3):461-7. doi: 10.1007/s10534-008-9182-3. Epub 2008 Dec 12.
Larvae of the insect Galleria mellonella were employed to assess the in vivo antifungal efficacy of ([Ag(2)(mal)(phen)(3)]), AgNO(3) and 1,10-phenanthroline. Larvae pre-inoculated with these compounds were protected from a subsequent lethal infection by the yeast Candida albicans while larvae inoculated 1 and 4 h post-infection showed significantly increased survival (P < 0.01) compared to control larvae. Administration of these compounds resulted in an increase over 48 h in the density of insect haemocytes (immune cells) but there was no widespread activation of genes for antimicrobial peptides. This work demonstrates that G. mellonella larvae may be employed to ascertain the antifungal efficacy of silver(I) compounds and offers a rapid and effective means of assessing the in vivo activity of inorganic antimicrobial compounds.
利用大蜡螟幼虫评估了[Ag(2)(mal)(phen)(3)]、AgNO(3)和1,10 - 菲咯啉的体内抗真菌功效。预先接种这些化合物的幼虫可免受随后白色念珠菌致死性感染的侵害，而在感染后1小时和4小时接种的幼虫与对照幼虫相比，存活率显著提高（P < 0.01）。给予这些化合物导致昆虫血细胞（免疫细胞）密度在48小时内增加，但抗菌肽基因没有广泛激活。这项工作表明，大蜡螟幼虫可用于确定银（I）化合物的抗真菌功效，并提供了一种快速有效的评估无机抗菌化合物体内活性的方法。
