Department of Health and Medical Sciences "V. Tiberio", University of Molise Via de Sanctis 3, III Ed. Polifunzionale, 86100 Campobasso (CB) Molise, Italy.
Department of Agricultural, Environmental and Food Sciences (DiAAA), University of Molise, Via De Sanctis 3, III Ed. Polifunzionale, 86100 Campobasso, Italy.
Molecules. 2020 Oct 29;25(21):5010. doi: 10.3390/molecules25215010.
Berberine is an alkaloid of the protoberberine type used in traditional oriental medicine. Its biological activities include documented antibacterial properties against a wide variety of microorganisms; nonetheless, its use against strains isolated from urinary infections has not yet been widely investigated in vivo. The emergence of antimicrobial resistance requires new therapeutic approaches to ensure the continued effectiveness of antibiotics for the treatment and prevention of urinary infections. Moreover, uropathogenic (UPEC) has developed several virulence factors and resistance to routine antibiotic therapy. To this end, several in vitro and in vivo tests were conducted to assess the activity of berberine on uropathogenic strains. as an infection model was employed to confirm the in vivo translatability of in vitro data on berberine activity and its influence on adhesion and invasion proprieties of on human bladder cells. In vitro pre-treatment with berberine was able to decrease the adhesive and invasive UPEC ability. In vivo treatment increased the larvae survival infected with UPEC strains and reduced the number of circulating pathogens in larvae hemolymph. These preliminary findings demonstrated the efficacy and reliability of as in vivo model for pre-clinical studies of natural substances.
小檗碱是一种用于传统东方医学的原小檗碱型生物碱。其生物活性包括已被证实的对多种微生物的抗菌特性；然而，其在体内针对尿路感染分离株的应用尚未得到广泛研究。抗菌药物耐药性的出现需要新的治疗方法，以确保抗生素在治疗和预防尿路感染方面的持续有效性。此外，尿路致病性 （UPEC）已经发展出多种毒力因子和对常规抗生素治疗的耐药性。为此，进行了几项体外和体内试验来评估小檗碱对尿路致病性 株的活性。 使用 作为感染模型来确认小檗碱活性的体外数据在体内的可转化性及其对 黏附和侵袭人膀胱细胞特性的影响。体外用小檗碱预处理能够降低 UPEC 的黏附和侵袭能力。体内治疗增加了受 UPEC 菌株感染的幼虫的存活率，并减少了幼虫血淋巴中循环病原体的数量。这些初步研究结果证明了 作为临床前天然物质研究的体内模型的有效性和可靠性。
