Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan, University, Wuxi, 214122, Jiangsu, China.
Bioprocess Biosyst Eng. 2020 Sep;43(9):1639-1648. doi: 10.1007/s00449-020-02356-y. Epub 2020 Apr 29.
Lysozyme, a type of natural enzyme, has been widely used for bacteriostatic functionalization of various materials due to its efficient and selective antibacterial properties. Herein, we report the preparation and characterization of an eco-friendly antibacterial textile based on the immobilization of lysozyme from chicken egg white onto wool fibers. Tris(hydroxymethyl)phosphine (THP) was employed as the cross-linker for the immobilization of lysozyme on the surface of wool fiber. The mechanism of THP cross-linking was investigated via phosphorus test, energy-dispersive spectroscopy (EDX) and Fourier transform infrared spectroscopy (FT-IR). The surface staining, optimization of immobilization parameters, morphology, antibacterial properties, and durability of wool fibers with immobilized lysozyme were also assessed. The results show that hydroxymethyl groups of THP reacted with amino groups of wool fiber and lysozyme through Mannich reaction, which successfully immobilized lysozyme on the wool fiber. The wool fibers incorporated with lysozyme had better antibacterial properties and durability compared with the untreated wool fabric. This facile immobilization approach of lysozyme provides an effective strategy for environmentally benign modification and functionalization of keratin and keratin-containing materials.
溶菌酶是一种天然酶，由于其高效和选择性的抗菌特性，已被广泛用于各种材料的抑菌功能化。本文报道了一种基于鸡蛋白溶菌酶固定在羊毛纤维上的环保型抗菌纺织品的制备和表征。三羟甲基膦（THP）被用作将溶菌酶固定在羊毛纤维表面的交联剂。通过磷测试、能谱（EDX）和傅里叶变换红外光谱（FT-IR）研究了 THP 交联的机理。还评估了固定化溶菌酶的羊毛纤维的表面染色、固定化参数优化、形态、抗菌性能和耐久性。结果表明，THP 的羟甲基基团与羊毛纤维和溶菌酶的氨基通过曼尼希反应反应，成功地将溶菌酶固定在羊毛纤维上。与未经处理的羊毛织物相比，固定有溶菌酶的羊毛纤维具有更好的抗菌性能和耐久性。这种简单的溶菌酶固定方法为角蛋白和含角蛋白材料的环保改性和功能化提供了一种有效的策略。
