获得性免疫缺陷综合征患者血清中免疫球蛋白A亚类分布

Immunoglobulin A subclass distribution in serum from patients with the acquired immunodeficiency syndrome.

作者信息

Cassulis P, Farhangfar R, DeBari V A

机构信息

Department of Medicine, St. Joseph's Hospital and Medical Center, School of Graduate Medical Education, Paterson, NJ 07503.

出版信息

Clin Biochem. 1991 Jun;24(3):277-81. doi: 10.1016/0009-9120(91)80020-4.

DOI:10.1016/0009-9120(91)80020-4

Abstract

Immunoglobulin levels are elevated in the acquired immune deficiency syndrome (AIDS) and one class, IgA, has been suggested as a marker of disease progression. IgA is composed of two subclasses, IgA1 and IgA2; circulating IgA1 is roughly 85% of the total in healthy individuals. We have attempted to determine if the subclass distribution in the serum of AIDS patients is similar to that of healthy subjects. IgA subclasses were measured by end-point radial immunodiffusion (RID). Sera from 27 AIDS patients and 27 controls who approximated the age and sex composition of the AIDS group were studied. Total IgA by RID, i.e., the sum of IgA1 and IgA2, correlated well with IgA determined by rate nephelometry (n = 51; r = 0.918; p less than 0.0001). Total IgA (IgAT), IgA1 and IgA2 were significantly elevated in the AIDS group (p less than 0.0001, p = 0.0001 and p = 0.033, respectively). However, the AIDS patients had a roughly equivalent percent IgA1 of total (87.2 +/- 7.6%) as the control group (85.0 +/- 6.1%). The subclass distribution is not altered in AIDS patients, and serum concentrations of IgA subclasses do not reflect possible alterations in secretory IgA which might be expected because of recurrent mucosal infections in these patients; nor is the subclass distribution altered due to clearance of predominantly IgA1-bearing immune complexes.

摘要

在获得性免疫缺陷综合征（艾滋病）中免疫球蛋白水平会升高，其中一类，即免疫球蛋白A（IgA），被认为是疾病进展的标志物。IgA由两个亚类组成，即IgA1和IgA2；在健康个体中，循环中的IgA1约占总量的85%。我们试图确定艾滋病患者血清中的亚类分布是否与健康受试者相似。通过终点放射免疫扩散法（RID）测量IgA亚类。研究了27名艾滋病患者和27名年龄和性别构成与艾滋病组相近的对照者的血清。通过RID法测得的总IgA，即IgA1和IgA2之和，与通过速率散射比浊法测定的IgA相关性良好（n = 51；r = 0.918；p < 0.0001）。艾滋病组的总IgA（IgAT）、IgA1和IgA2均显著升高（分别为p < 0.0001、p = 0.0001和p = 0.033）。然而，艾滋病患者的IgA1占总量的百分比（87.2±7.6%）与对照组（85.0±6.1%）大致相当。艾滋病患者的亚类分布没有改变，并且IgA亚类的血清浓度并不能反映分泌型IgA可能的变化，而由于这些患者反复发生黏膜感染，分泌型IgA可能会发生变化；亚类分布也不会因主要携带IgA1的免疫复合物的清除而改变。