用于降解DNA样本分析的短扩增子长度MiniY-STR四重系统。
MiniY-STR quadruplex systems with short amplicon lengths for analysis of degraded DNA samples.
作者信息
Asamura H, Sakai H, Ota M, Fukushima H
机构信息
Department of Legal Medicine, Shinshu University School of Medicine, Japan.
出版信息
Forensic Sci Int Genet. 2007 Mar;1(1):56-61. doi: 10.1016/j.fsigen.2006.10.008. Epub 2006 Nov 28.
Two short amplicon Y-chromosomal short tandem repeat (miniY-STR) quadruplex systems for the eight Y-STR loci DYS522, DYS508, DYS632, DYS556, DYS570, DYS576, DYS504 and DYS540 were devised using newly designed primer sets. Among 224 samples from Japanese population, amplification product lengths detected in these Y-STR loci ranged from 95 to 147bp, while 170 different haplotype were identified (discrimination capacity=0.7589 and haplotype diversity=0.9949). As a result of test on degraded DNA samples using the miniY-STR quadruplex systems, the systems proved to be an quite effective tools for analyzing degraded DNAs. We conclude that analyses of the miniY-STR quadruplex systems in addition to the commercial available Y-STR multiplex kits are highly useful for forensic practices of degraded DNA samples.
利用新设计的引物组，设计了用于8个Y染色体短串联重复序列（miniY-STR）位点DYS522、DYS508、DYS632、DYS556、DYS570、DYS576、DYS504和DYS540的两个短扩增子Y染色体短串联重复序列四重体系。在来自日本人群的224个样本中，这些Y-STR位点检测到的扩增产物长度在95至147bp之间，同时鉴定出170种不同的单倍型（鉴别能力=0.7589，单倍型多样性=0.9949）。使用miniY-STR四重体系对降解DNA样本进行测试的结果表明，该体系是分析降解DNA的非常有效的工具。我们得出结论，除了市售的Y-STR多重试剂盒外，miniY-STR四重体系的分析对于降解DNA样本的法医实践非常有用。
Zotero 插件