委内瑞拉加拉加斯市人类线粒体DNA高变区I-III的单倍型多样性
Haplotype diversity in human mitochondrial DNA hypervariable regions I-III in the city of Caracas (Venezuela).
作者信息
Lander N, Rojas M G, Chiurillo M A, Ramírez J L
机构信息
Centro de Biotecnología, Fundación Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela.
出版信息
Forensic Sci Int Genet. 2008 Sep;2(4):e61-4. doi: 10.1016/j.fsigen.2007.12.009. Epub 2008 Feb 14.
In order to expand the database of variable DNA for forensic identification purposes in Venezuela, we analyzed the sequence polymorphisms of mitochondrial DNA (mtDNA) hypervariable regions (HVR) I-III from 100 unrelated individuals from the city of Caracas, using PCR amplification and fluorescent-based capillary electrophoresis sequencing method. Dominant haplogroups corresponded to Native Americans followed by African ones. The inclusion of HVR III although useful for sub-haplogroup assignation, added little to the discrimination capacity of our database.
为了扩充委内瑞拉用于法医鉴定的可变DNA数据库，我们采用聚合酶链反应（PCR）扩增和基于荧光的毛细管电泳测序方法，分析了来自加拉加斯市100名无亲缘关系个体的线粒体DNA（mtDNA）高变区（HVR）I - III的序列多态性。主要单倍群对应美洲原住民，其次是非洲裔单倍群。尽管HVR III对于亚单倍群的划分有用，但对我们数据库的鉴别能力提升不大。
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