巴西亚马逊地区非洲裔人群中HV-I的核苷酸变异性
Nucleotide variability of HV-I in Afro-descendents populations of the Brazilian Amazon Region.
作者信息
Ribeiro-dos-Santos Andrea Kely Campos, Carvalho Bruno Maia, Feio-dos-Santos Ana Cecília, dos Santos Sidney Emanuel Batista
机构信息
Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brasil.
出版信息
Forensic Sci Int. 2007 Mar 22;167(1):77-80. doi: 10.1016/j.forsciint.2005.12.033. Epub 2006 Jan 31.
The analysis of genetic variation in the nucleotide sequences of mitochondrial DNA, provides unique information about the population diversity and human identification. In this study, the mitochondrial DNA sequences of the first hypervariable region (HV-I) were analyzed in 243 unrelated individuals of seven Afro-descendents populations of the Amazon Region. Sequence polymorphisms were detected using PCR and direct sequencing analysis. A total of 133 different haplotypes were found determined by 97 variable nucleotides. Each one of the three more frequent haplotypes was shared by 9 samples and 91 sequences were unique. The genetic diversity was estimated to 0.9898+/-0.0016 and the probability of two random individuals showed identical mitochondrial DNA (mtDNA) haplotypes were 1.2%.
对线粒体DNA核苷酸序列中的遗传变异进行分析，可为群体多样性和人类识别提供独特信息。在本研究中，对亚马逊地区七个非洲裔群体的243名无亲缘关系个体的线粒体DNA第一高变区（HV-I）序列进行了分析。使用PCR和直接测序分析检测序列多态性。通过97个可变核苷酸确定共发现了133种不同单倍型。三种最常见的单倍型中每种都有9个样本共享，91个序列是独特的。估计遗传多样性为0.9898±0.0016，两个随机个体具有相同线粒体DNA（mtDNA）单倍型的概率为1.2%。
Zotero 插件