205名新加坡马来人的线粒体DNA高变区I和II的序列多态性
Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays.
作者信息
Wong Hang Yee, Tang June S W, Budowle Bruce, Allard Marc W, Syn Christopher K C, Tan-Siew Wai Fun, Chow Shui Tse
机构信息
DNA Profiling Laboratory, Centre for Forensic Science, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore.
出版信息
Leg Med (Tokyo). 2007 Jan;9(1):33-7. doi: 10.1016/j.legalmed.2006.08.007. Epub 2006 Dec 5.
Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively.
对居住在新加坡的205名无血缘关系的马来族个体的线粒体DNA高变区HV1和HV2序列进行了分析，作为建立用于法医鉴定目的数据库的初步工作。使用聚合酶链反应（PCR）和直接测序分析检测序列多态性。共发现152种单倍型，包含152种多态性。在这152种单倍型中，115种仅出现一次，37种在多个个体中出现。最常见的单倍型（16223T、16295T、16362C、73G、146C、199C、263G和315.1C）有7名个体（3.41%）共享，两种单倍型由4名个体共享，七种单倍型由3名个体共享，27种单倍型由2名个体共享。单倍型多样性和随机匹配概率估计分别为0.9961%和0.87%。
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