Killough Simon A, Lundy Fionnuala T, Irwin Chris R
Department of Restorative Dentistry, School of Dentistry, Royal Group of Hospitals, Queen's University Belfast, Belfast, United Kingdom.
J Endod. 2009 Jan;35(1):73-7. doi: 10.1016/j.joen.2008.10.010.
Neurogenic inflammation describes the local release of neuropeptides, notably substance P (SP), from afferent neurons and might play a role in the pathogenesis of pulpal disease. The fibroblast is the most numerous cell type in the dental pulp, and recent work has suggested that it is involved in the inflammatory response. Primary pulp fibroblast cell populations were isolated by enzymatic digestion. Whole pulp tissue was obtained from freshly extracted sound (n = 35) and carious (n = 39) teeth. Expression of SP and neurokinin-1 receptor (NK-1) mRNA by pulp fibroblasts was determined by reverse transcriptase polymerase chain reaction (RT-PCR). SP was expressed by pulpal fibroblasts at both mRNA and protein levels. In addition, NK-1 mRNA and protein expression was detected in fibroblast cultures by RT-PCR and Western blotting, respectively. SP levels, determined by radioimmunoassay, were significantly greater (P < .05) in carious compared with sound teeth. These findings suggest that pulp fibroblasts play a role in neurogenic inflammation in pulpal disease.
神经源性炎症是指传入神经元局部释放神经肽，尤其是P物质（SP），它可能在牙髓病的发病机制中起作用。成纤维细胞是牙髓中数量最多的细胞类型，最近的研究表明它参与了炎症反应。通过酶消化分离出原代牙髓成纤维细胞群体。从新鲜拔除的健康牙齿（n = 35）和龋坏牙齿（n = 39）中获取全牙髓组织。通过逆转录聚合酶链反应（RT-PCR）测定牙髓成纤维细胞中SP和神经激肽-1受体（NK-1）mRNA的表达。牙髓成纤维细胞在mRNA和蛋白质水平均表达SP。此外，分别通过RT-PCR和蛋白质印迹法在成纤维细胞培养物中检测到NK-1 mRNA和蛋白质表达。通过放射免疫测定法测定，龋坏牙齿中的SP水平明显高于健康牙齿（P <.05）。这些发现表明牙髓成纤维细胞在牙髓病的神经源性炎症中起作用。
