Williamson Anne E, Cardon Jared W, Drake David R
Department of Endodontics, University of Iowa College of Dentistry, Iowa City, Iowa, USA.
J Endod. 2009 Jan;35(1):95-7. doi: 10.1016/j.joen.2008.09.004. Epub 2008 Nov 7.
The purpose of this study was to create a monoculture biofilm of a clinical isolate of Enterococcus faecalis and to determine susceptibility against four antimicrobial irrigants. Biofilms were subjected to 1-, 3-, and 5-minute exposures to one of the following irrigants: 6% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX) or one of two new products, <6% NaOCl with surface modifiers (Chlor-XTRA) or 2% CHX with surface modifiers (CHX-Plus) (Vista Dental Products, Racine, WI). It was hypothesized that NaOCl and CHX would be equally effective and that addition of surface modifiers would improve bactericidal activity of the respective irrigants compared to the original formulations. Results indicate that 6% NaOCl and Chlor-EXTRA were significantly superior against E. faecalis biolfilms compared to 2% CHX and CHX-Plus at all time points except five minutes.
本研究的目的是创建粪肠球菌临床分离株的单培养生物膜，并确定其对四种抗菌冲洗剂的敏感性。将生物膜分别暴露于以下冲洗剂之一1分钟、3分钟和5分钟：6%次氯酸钠（NaOCl）、2%葡萄糖酸氯己定（CHX）或两种新产品之一，含表面改性剂的<6% NaOCl（Chlor-XTRA）或含表面改性剂的2% CHX（CHX-Plus）（维斯塔牙科产品公司，威斯康星州拉辛）。研究假设是，NaOCl和CHX的效果相同，并且与原始配方相比，添加表面改性剂将提高各自冲洗剂的杀菌活性。结果表明，除了5分钟时，在所有时间点，6% NaOCl和Chlor-XTRA对粪肠球菌生物膜的杀菌效果均显著优于2% CHX和CHX-Plus。
