根管冲洗剂对粪肠球菌生物膜的比较评价

Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms.

作者信息

Dunavant Thomas R, Regan John D, Glickman Gerald N, Solomon Eric S, Honeyman Allen L

机构信息

Department of Endodontics, Baylor College of Dentistry, The Texas A&M University System Health Sciences Center, Dallas, Texas 75246, USA.

出版信息

J Endod. 2006 Jun;32(6):527-31. doi: 10.1016/j.joen.2005.09.001.

DOI:10.1016/j.joen.2005.09.001

PMID:16728243

Abstract

The aim of this study was to compare the efficacy of root canal irrigants against E. faecalis biofilms using a novel in vitro testing system. Biofilms grown in a flow cell system were submerged in test irrigants for either 1 or 5 minutes. Statistical analysis revealed a significant relationship between test agent and percentage kill of the biofilm bacteria (P < 0.05). No statistically significant relationship between time and percentage kill was found. The percentage kill of the biofilm bacteria was: 6% NaOCl (>99.99%), 1% NaOCl (99.78%), Smear Clear (78.06%), 2% chlorhexidine (60.49%), REDTA (26.99%), and BioPure MTAD (16.08%). Post-hoc analysis showed a significant difference between 1% and 6% NaOCl, and all other agents including Smear Clear, 2% chlorhexidine, REDTA, and BioPure MTAD (P < 0.05). Within the parameters of this study, both 1% NaOCl and 6% NaOCl were more efficient in eliminating E. faecalis biofilm than the other solutions tested.

摘要

本研究的目的是使用一种新型体外测试系统，比较根管冲洗剂对粪肠球菌生物膜的疗效。在流动池系统中生长的生物膜被浸泡在测试冲洗剂中1分钟或5分钟。统计分析显示测试剂与生物膜细菌杀灭率之间存在显著关系（P < 0.05）。未发现时间与杀灭率之间存在统计学上的显著关系。生物膜细菌的杀灭率分别为：6%次氯酸钠（>99.99%）、1%次氯酸钠（99.78%）、Smear Clear（78.06%）、2%氯己定（60.49%）、REDTA（26.99%）和BioPure MTAD（16.08%）。事后分析显示1%和6%次氯酸钠之间以及与所有其他试剂（包括Smear Clear、2%氯己定、REDTA和BioPure MTAD）之间存在显著差异（P < 0.05）。在本研究的参数范围内，1%次氯酸钠和6%次氯酸钠在消除粪肠球菌生物膜方面比其他测试溶液更有效。