Saha Suchismita, Kaviraj Anilava
Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India.
Chemosphere. 2009 Mar;74(9):1254-9. doi: 10.1016/j.chemosphere.2008.10.056. Epub 2008 Dec 11.
Static bioassays were made to evaluate efficiency of dietary supplementation of ascorbic acid to counter stress exerted by the pyrethroid pesticide, cypermethrin on freshwater catfish Heteropneustes fossilis. Three levels, a control and two sub-lethal concentrations (0.3 and 0.5 microg L(-1)) of cypermethrin were tested. Stress on H. fossilis was evident from the rise of plasma glucose level and reduction in the level of liver glycogen after 4 h of cypermethrin treatment. Acid and alkaline phosphatase activities of liver and ascorbic acid level of blood, liver and kidney also decreased significantly in H. fossilis exposed to cypermethrin as compared with control. Fish previously fed for 60 d with a diet supplemented by high level of ascorbic acid (1.0 g kg(-1)) could significantly reverse these effects. Fish fed a low level of dietary ascorbic acid (0.5 g kg(-1)) failed to counter the stress of cypermethrin.
进行了静态生物测定，以评估在饲料中添加抗坏血酸对抗拟除虫菊酯类杀虫剂氯氰菊酯对淡水鲶鱼（印度胡鲶）施加的应激的效果。测试了三个水平，一个对照以及氯氰菊酯的两个亚致死浓度（0.3和0.5微克/升）。在氯氰菊酯处理4小时后，印度胡鲶血浆葡萄糖水平升高以及肝糖原水平降低，表明其受到了应激。与对照组相比，暴露于氯氰菊酯的印度胡鲶肝脏的酸性和碱性磷酸酶活性以及血液、肝脏和肾脏中的抗坏血酸水平也显著降低。预先用高剂量抗坏血酸（1.0克/千克）补充饲料喂养60天的鱼能够显著逆转这些影响。用低剂量抗坏血酸（0.5克/千克）喂养的鱼未能抵抗氯氰菊酯的应激。
