Schrøder Merete Bjørgan, Ellingsen Terje, Mikkelsen Helene, Norderhus Edel Anne, Lund Vera
Norwegian College of Fishery Science, University of Tromsø, Breivika, Tromso N-9037, Norway.
Fish Shellfish Immunol. 2009 Aug;27(2):112-9. doi: 10.1016/j.fsi.2008.11.016. Epub 2008 Dec 6.
Bacterial diseases such as vibriosis, atypical furunculosis and francisellosis, are registered as an increasing problem in cod farming in Norway. In order to develop efficient vaccines against diseases it is of interest to investigate if the cod immune system differentiates between various serotypes of Vibrio anguillarum and variants of Aeromonas salmonicida associated with the diseases by raising specific antibody responses. Cod of the same origin were shown to raise significant responses to V. anguillarum, A. salmonicida and the intracellular bacteria Francisella sp. Individual responses to V. anguillarum or A. salmonicida varied from none to high responses, while all individuals immunised with Francisella revealed a significant response. The cod immune system appeared in some degree to distinguish between V. anguillarum serotypes and A. salmonicida variants. Although all bacteria had induced significant antibody responses detectable in whole cell ELISA, only some had induced antibodies with specificity to linear O-polysaccharide epitopes on blot.
诸如弧菌病、非典型疖病和弗朗西斯菌病等细菌性疾病，在挪威鳕鱼养殖中已成为一个日益严重的问题。为了研发针对这些疾病的有效疫苗，研究鳕鱼免疫系统是否通过产生特异性抗体反应来区分鳗弧菌的不同血清型以及与这些疾病相关的杀鲑气单胞菌变体，是很有意义的。研究表明，同一来源的鳕鱼对鳗弧菌、杀鲑气单胞菌和细胞内细菌弗朗西斯菌属产生了显著反应。个体对鳗弧菌或杀鲑气单胞菌的反应从无反应到高反应各不相同，而所有用弗朗西斯菌免疫的个体都显示出显著反应。鳕鱼免疫系统在一定程度上似乎能够区分鳗弧菌血清型和杀鲑气单胞菌变体。尽管所有细菌在全细胞酶联免疫吸附测定中都诱导产生了可检测到的显著抗体反应，但只有一些细菌在印迹法中诱导产生了针对线性O - 多糖表位的特异性抗体。
