Tada Rui, Adachi Yoshiyuki, Ishibashi Ken-ichi, Ohno Naohito
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Carbohydr Res. 2009 Feb 17;344(3):400-4. doi: 10.1016/j.carres.2008.11.005. Epub 2008 Nov 13.
Grifolan LE (GRN-LE), a purified beta-D-glucan, which is obtained from liquid-cultured Grifola frondosa, exhibits various biological activities, including antitumor effects. Significant progress has been made in the study of these effects. However, an unambiguous structural characterization of GRN-LE using NMR spectroscopy has not been carried out as yet. It is well accepted that the biological effects of a beta-glucan depend on its primary structure, conformation, and molecular weight. In the present study, we unambiguously elucidate the primary structure of GRN-LE using NMR spectroscopy. The data presented here reveal that GRN-LE comprises a 1,3-beta-D-glucan backbone with a single 1,6-beta-D-glucosyl side branching unit on every third residue.
灰树花多糖 LE（GRN-LE）是一种从液体培养的灰树花中获得的纯化β-D-葡聚糖，具有多种生物活性，包括抗肿瘤作用。在这些作用的研究方面已经取得了重大进展。然而，尚未使用核磁共振光谱对 GRN-LE 进行明确的结构表征。人们普遍认为，β-葡聚糖的生物学效应取决于其一级结构、构象和分子量。在本研究中，我们使用核磁共振光谱明确阐明了 GRN-LE 的一级结构。此处给出的数据表明，GRN-LE 由一个 1,3-β-D-葡聚糖主链组成，每三个残基上有一个单一的 1,6-β-D-葡萄糖基侧分支单元。
