Tada Rui, Harada Toshie, Nagi-Miura Noriko, Adachi Yoshiyuki, Nakajima Mitsuhiro, Yadomae Toshiro, Ohno Naohito
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan.
Carbohydr Res. 2007 Dec 10;342(17):2611-8. doi: 10.1016/j.carres.2007.08.016. Epub 2007 Aug 31.
SCG, a purified beta-d-glucan, obtained from Sparassis crispa, exhibits various biological activities including an antitumor effect, enhancement of the hematopoietic response in cyclophosphamide-induced leukopenic mice, and induction of the production of cytokines. The mechanisms of these effects have been extensively investigated; however, an unambiguous structural characterization of SCG is yet to be achieved. It is well accepted that the biological effects of beta-glucan depend on its primary structures, conformation, and molecular weight. In the present study, we examine the difference of biological effects among beta-glucans, elucidate the primary structure of SCG, and compare with SPG from Schizophyllum commune using NMR spectroscopy. Our data reveal that SCG but not SPG induce cytokine production from bone marrow-derived dendritic cells (BMDCs) and their major structural units are a beta-(1-->3)-d-glucan backbone with single beta-(1-->6)-d-glucosyl side branching units every three residues.
SCG是一种从皱盖乌芝中获得的纯化β - d - 葡聚糖，具有多种生物学活性，包括抗肿瘤作用、增强环磷酰胺诱导的白细胞减少小鼠的造血反应以及诱导细胞因子的产生。这些作用的机制已得到广泛研究；然而，SCG的明确结构表征尚未实现。人们普遍认为，β - 葡聚糖的生物学效应取决于其一级结构、构象和分子量。在本研究中，我们研究了β - 葡聚糖之间生物学效应的差异，阐明了SCG的一级结构，并使用核磁共振光谱与裂褶菌的SPG进行比较。我们的数据显示，SCG而非SPG可诱导骨髓来源的树突状细胞（BMDCs）产生细胞因子，其主要结构单元是每三个残基带有单个β - (1→6) - d - 葡糖基侧支链单元的β - (1→3) - d - 葡聚糖主链。
