Rubera Isabelle, Hummler Edith, Beermann Friedrich
CNRS-FRE, Université de Nice Sophia-Antipolis, France.
Pflugers Arch. 2009 May;458(1):211-22. doi: 10.1007/s00424-008-0624-0. Epub 2008 Dec 16.
The kidney is a key organ in the maintenance of ion and fluid homeostasis and specific transport systems localized along the nephron guarantee this function. Due to its large functional heterogeneity, experiments on the whole organ level cannot be easily performed, and thus more refined tools are needed, like for example the development of specific recombination systems to gain knowledge on the physiological role of single proteins implicated in ion transport. This review introduces the transgenic technology developed over the past decades, and then focuses on recent strategies for generating kidney-specific gene targeting, over-expression, and gene ablation in mice, that will help to understand the physiological role of proteins implicated in salt and water balance in the kidney.
肾脏是维持离子和液体稳态的关键器官，沿肾单位定位的特定转运系统保证了这一功能。由于其功能异质性大，在整个器官水平上进行实验并不容易，因此需要更精细的工具，例如开发特定的重组系统，以了解参与离子转运的单个蛋白质的生理作用。本文综述了过去几十年发展起来的转基因技术，然后重点介绍了在小鼠中产生肾脏特异性基因靶向、过表达和基因敲除的最新策略，这将有助于理解参与肾脏盐和水平衡的蛋白质的生理作用。
