通过注射实现秀丽隐杆线虫的种系转化。
Germline transformation of Caenorhabditis elegans by injection.
作者信息
Kadandale Pavan, Chatterjee Indrani, Singson Andrew
机构信息
Waksman Institute, Rutgers University, Piscataway, NJ, USA.
出版信息
Methods Mol Biol. 2009;518:123-33. doi: 10.1007/978-1-59745-202-1_10.
Abstract
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.
摘要
显微注射是秀丽隐杆线虫中常用的DNA转化技术。它是一种将遗传和分子分析与表型分析联系起来的强大工具。在本章中，我们将概述线虫种系转化的显微注射方法。我们的讨论将重点关注秀丽隐杆线虫的生殖生物学、进行显微注射以成功获得转基因线虫的应用和方案。
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本文引用的文献
1
Integrative transformation of Caenorhabditis elegans.秀丽隐杆线虫的综合转化。
EMBO J. 1986 Oct;5(10):2673-80. doi: 10.1002/j.1460-2075.1986.tb04550.x.
2
Investigating C. elegans development through mosaic analysis.通过镶嵌分析研究秀丽隐杆线虫的发育。
Development. 2003 Oct;130(20):4761-8. doi: 10.1242/dev.00701.
3
Creation of low-copy integrated transgenic lines in Caenorhabditis elegans.秀丽隐杆线虫中低拷贝整合转基因系的创建。
Genetics. 2001 Mar;157(3):1217-26. doi: 10.1093/genetics/157.3.1217.
4
Distinct requirements for somatic and germline expression of a generally expressed Caernorhabditis elegans gene.普遍表达的秀丽隐杆线虫基因的体细胞和生殖系表达的不同要求。
Genetics. 1997 May;146(1):227-38. doi: 10.1093/genetics/146.1.227.
5
DNA transformation.DNA转化
Methods Cell Biol. 1995;48:451-82.
6
Suppression of an amber mutation by microinjection of suppressor tRNA in C. elegans.通过向秀丽隐杆线虫显微注射抑制性tRNA来抑制琥珀突变。
Nature. 1982 Sep 30;299(5882):456-8. doi: 10.1038/299456a0.
7
The genetics of Caenorhabditis elegans.秀丽隐杆线虫的遗传学
Genetics. 1974 May;77(1):71-94. doi: 10.1093/genetics/77.1.71.
8
Extrachromosomal DNA transformation of Caenorhabditis elegans.秀丽隐杆线虫的染色体外DNA转化
Mol Cell Biol. 1985 Dec;5(12):3484-96. doi: 10.1128/mcb.5.12.3484-3496.1985.
9
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.秀丽隐杆线虫中的高效基因转移：转化序列的染色体外维持与整合
EMBO J. 1991 Dec;10(12):3959-70. doi: 10.1002/j.1460-2075.1991.tb04966.x.
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