Kadandale Pavan, Chatterjee Indrani, Singson Andrew
Waksman Institute, Rutgers University, Piscataway, NJ, USA.
Methods Mol Biol. 2009;518:123-33. doi: 10.1007/978-1-59745-202-1_10.
Microinjection is a commonly used technique for DNA transformation in Caenorhabditis elegans. It is a powerful tool that links genetic and molecular analysis to phenotypic analysis. In this chapter we shall provide an overview of microinjection for germline transformation in worms. Our discussion will emphasize C. elegans reproductive biology, applications and protocols for carrying out microinjection in order to successfully obtain transgenic worms.
显微注射是秀丽隐杆线虫中常用的DNA转化技术。它是一种将遗传和分子分析与表型分析联系起来的强大工具。在本章中，我们将概述线虫种系转化的显微注射方法。我们的讨论将重点关注秀丽隐杆线虫的生殖生物学、进行显微注射以成功获得转基因线虫的应用和方案。
