Algeciras Mabel E, Takahara Hidenari, Bhattacharya Sanjoy K
Bascom Palmer Eye Institute, University of Miami, Miami, Florida, USA.
Curr Eye Res. 2008 Nov;33(11):994-1001. doi: 10.1080/02713680802447113.
To determine whether the mechanical stretching renders modulation of the peptidyl arginine deiminase 2 (PAD2) expression in cultured astrocytes.
Isolated rat brain astrocytes were subjected to mechanical stretching using a glass bead set-up and polyethylene set-up with or without immobilization. Activity assays and ELISA were performed to detect PAD2 expression. Protein deimination levels in the cells were measured using an anti-citrulline ELISA. PAD2 expression in cells subjected to mechanical stretching was compared with controls. Astrocytes were also subjected to pressure treatment and compared to control cells for PAD2 expression and deimination levels.
Astrocytes subjected to mechanical stretching with or without immobilization showed elevated PAD2 expression. Pressure treatment of astrocytes (25-100 mmHg) also resulted in elevated PAD2 expression and deimination.
These results suggest mechanical stretching induced PAD2 expression and consequent protein deimination.
确定机械拉伸是否会调节培养星形胶质细胞中肽基精氨酸脱亚氨酶2（PAD2）的表达。
使用玻璃珠装置和聚乙烯装置对分离的大鼠脑星形胶质细胞进行机械拉伸，有或没有固定。进行活性测定和酶联免疫吸附测定（ELISA）以检测PAD2的表达。使用抗瓜氨酸ELISA测量细胞中的蛋白质脱亚胺水平。将进行机械拉伸的细胞中的PAD2表达与对照进行比较。还对星形胶质细胞进行压力处理，并与对照细胞比较PAD2表达和脱亚胺水平。
进行了有或没有固定的机械拉伸的星形胶质细胞显示出PAD2表达升高。对星形胶质细胞进行压力处理（25 - 100毫米汞柱）也导致PAD2表达和脱亚胺升高。
这些结果表明机械拉伸诱导了PAD2表达以及随之而来的蛋白质脱亚胺。
