Kremling Andreas, Kremling Sophia, Bettenbrock Katja
Systems Biology Group, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
FEBS J. 2009 Jan;276(2):594-602. doi: 10.1111/j.1742-4658.2008.06810.x. Epub 2008 Dec 11.
The phosphotransferase system in Escherichia coli is a transport and sensory system and, in this function, is one of the key players of catabolite repression. Mathematical modelling of signal transduction and gene expression of the enzymes involved in the transport of carbohydrates is a promising approach in biotechnology, as it offers the possibility to achieve higher production rates of desired components. In this article, the relevance of methods and approaches concerning mathematical modelling in systems biology is discussed by assessing and comparing two comprehensive mathematical models that describe catabolite repression. The focus is thereby on modular modelling with the relevant input in the central modules, the impact of quantitative model validation, the identification of control structures and the comparison of model predictions with respect to the available experimental data.
大肠杆菌中的磷酸转移酶系统是一种运输和传感系统，在这一功能中，它是分解代谢物阻遏的关键参与者之一。对参与碳水化合物运输的酶的信号转导和基因表达进行数学建模是生物技术中一种很有前景的方法，因为它提供了实现更高产量所需成分的可能性。在本文中，通过评估和比较两个描述分解代谢物阻遏的综合数学模型，讨论了系统生物学中数学建模方法和途径的相关性。因此，重点在于中央模块中相关输入的模块化建模、定量模型验证的影响、控制结构的识别以及模型预测与现有实验数据的比较。
