Genetics of Prokaryotes, Faculty of Biology & CeBiTec, University of Bielefeld, 33615 Bielefeld, Germany.
Comput Struct Biotechnol J. 2012 Oct 30;3:e201210004. doi: 10.5936/csbj.201210004. eCollection 2012.
Corynebacterium glutamicum is well known as the amino acid-producing workhorse of fermentation industry, being used for multi-million-ton scale production of glutamate and lysine for more than 60 years. However, it is only recently that extensive research has focused on engineering it beyond the scope of amino acids. Meanwhile, a variety of corynebacterial strains allows access to alternative carbon sources and/or allows production of a wide range of industrially relevant compounds. Some of these efforts set new standards in terms of titers and productivities achieved whereas others represent a proof-of-principle. These achievements manifest the position of C. glutamicum as an important industrial microorganism with capabilities far beyond the traditional amino acid production. In this review we focus on the state of the art of metabolic engineering of C. glutamicum for utilization of alternative carbon sources, (e.g. coming from wastes and unprocessed sources), and construction of C. glutamicum strains for production of new products such as diamines, organic acids and alcohols.
谷氨酸棒杆菌是发酵工业中生产氨基酸的重要生产菌，其用于生产谷氨酸和赖氨酸的规模已达数百万吨，至今已有超过 60 年的历史。然而，直到最近，人们才开始广泛研究如何在氨基酸生产以外的领域对其进行工程改造。同时，多种棒杆菌菌株可用于替代碳源，或者可以生产广泛的工业相关化合物。其中一些研究成果在达到的产量和生产率方面设定了新的标准，而另一些则代表了原理验证。这些成果体现了谷氨酸棒杆菌作为一种重要的工业微生物的地位，其能力远远超出了传统的氨基酸生产。在本综述中，我们重点介绍了谷氨酸棒杆菌利用替代碳源（例如来自废物和未加工来源的碳源）的代谢工程的最新进展，以及构建用于生产新产物（例如二胺、有机酸和醇）的谷氨酸棒杆菌菌株的进展。
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