[三氧化矿物凝聚体与氢氧化钙对乳牙牙髓细胞增殖及分化能力的影响]

[Effects of mineral trioxide aggregate and calcium hydroxide on the proliferation and differentiation capacity of pulp cells of primary teeth].

作者信息

Wang Min-yong, Liu He, Li Sheng-lin, Qin Man

机构信息

Research Laboratory of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 2008 Sep;43(9):524-7.

DOI:

PMID:19087601

Abstract

OBJECTIVE

To investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on the proliferation and differentiation capacity of pulp cells of primary teeth.

METHODS

Pulp cells were isolated from the retained primary teeth without apparent root resorption and cultured. The cells of 4 - 8 passages were used in the study. Cell proliferation was detected by MTT array, von Kossa staining employed to observe the formation of mineralized nodules and mRNA expression of alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP) determined by real time PCR.

RESULTS

MTA-treated cells proliferated significantly faster than the other two groups (F = 1835.065, P < 0.01), while calcium hydroxide-treated cells grew slower than the control significantly (F = 1792.301, P < 0.01). The formation of mineralized nodules was found in both MTA-treated and calcium hydroxide-treated pulp cells. The number of mineralized nodules showed no significant difference between the two groups (P > 0.05). Either ALP or DSPP mRNA expression showed significant difference among the three groups (F = 349.651, P < 0.01; F = 1653.001, P < 0.01). MTA increased mRNA expression of ALP and DSPP in pulp cells (P < 0.01), whereas calcium hydroxide down-regulated them (P < 0.01).

CONCLUSIONS

MTA is more suitable than calcium hydroxide as pulp-capping agent in primary teeth.

摘要

目的

研究三氧化矿物凝聚体（MTA）和氢氧化钙对乳牙髓细胞增殖及分化能力的影响。

方法

从无明显牙根吸收的滞留乳牙中分离培养牙髓细胞。研究采用4 - 8代细胞。通过MTT法检测细胞增殖，用von Kossa染色观察矿化结节形成，采用实时PCR测定碱性磷酸酶（ALP）和牙本质涎磷蛋白（DSPP）的mRNA表达。

结果

MTA处理组细胞增殖明显快于其他两组（F = 1835.065，P < 0.01），而氢氧化钙处理组细胞生长明显慢于对照组（F = 1792.301，P < 0.01）。MTA处理组和氢氧化钙处理组的牙髓细胞均有矿化结节形成。两组矿化结节数量无显著差异（P > 0.05）。三组间ALP或DSPP的mRNA表达均有显著差异（F = 349.651，P < 0.01；F = 1653.001，P < 0.01）。MTA可增加牙髓细胞中ALP和DSPP的mRNA表达（P < 0.01），而氢氧化钙则下调其表达（P < 0.01）。