Department of Endodontics, Yeditepe University, Istanbul, Turkey.
J Endod. 2011 May;37(5):650-6. doi: 10.1016/j.joen.2011.02.008. Epub 2011 Mar 24.
Biocompatibility of pulp capping materials is important for successful use in dentistry. These materials should be nontoxic and permissive for proliferation and induction of odontogenic differentiation of pulp cells. The aim of our study was to evaluate the effects of enamel matrix derivative (EMD), mineral trioxide aggregate (MTA), and calcium hydroxide-containing cement (DYCAL) on proliferation and odontogenic differentiation of human tooth germ stem cells (hTGSCs) in which cells belonging to both pulp tissue and dental follicle exist.
The 96-well plates, 24-well plates, and special chamber slides were coated with biomaterials for cell proliferation, differentiation, and scanning electron microscopy analysis. Odontogenic differentiation of hTGSCs was evaluated by analyzing mRNA expression of dentin sialophosphoprotein (DSPP) by real-time polymerase chain reaction expression analysis, measurement of alkaline phosphatase activity, and visualization of calcium depositions by von Kossa staining.
Our results demonstrate that EMD is the best material in terms of inducing differentiation and proliferation of hTGSCs. DYCAL was found to be toxic to hTGSCs; however, EMD-coated DYCAL showed less toxicity. EMD-coated MTA was not efficient at inducing proliferation and differentiation.
Pulp capping materials come in direct contact with dental pulp cells; thus, they require comprehensive evaluation of interactions between cells and biomaterials. Therefore, we cultured hTGSCs, capable of odontogenic differentiation, on pulp capping materials directly. Our results suggest that combination of capping materials with EMD would increase the quality of capping by increasing biocompatibility of capping materials.
牙髓覆盖材料的生物相容性对于在牙科中的成功应用非常重要。这些材料应该是无毒的，并且允许牙髓细胞增殖和诱导牙源性分化。我们的研究目的是评估釉基质衍生物（EMD）、三氧化矿物聚合体（MTA）和含氢氧化钙的水泥（DYCAL）对牙髓干细胞（hTGSCs）增殖和牙源性分化的影响，这些细胞既存在于牙髓组织中，也存在于牙囊组织中。
将生物材料涂覆于 96 孔板、24 孔板和特殊室载玻片上，用于细胞增殖、分化和扫描电子显微镜分析。通过实时聚合酶链反应表达分析、碱性磷酸酶活性测量和 von Kossa 染色观察钙沉积，分析牙本质涎磷蛋白（DSPP）的 mRNA 表达，评估 hTGSCs 的牙源性分化。
我们的结果表明，在诱导 hTGSCs 分化和增殖方面，EMD 是最好的材料。DYCAL 对 hTGSCs 具有毒性，但 EMD 涂层的 DYCAL 毒性较小。EMD 涂层的 MTA 不能有效地诱导增殖和分化。
牙髓覆盖材料与牙髓细胞直接接触；因此，需要对细胞与生物材料之间的相互作用进行全面评估。因此，我们直接在牙髓覆盖材料上培养具有牙源性分化能力的 hTGSCs。我们的结果表明，将覆盖材料与 EMD 结合使用，通过提高覆盖材料的生物相容性，可提高覆盖的质量。
