迈向[Cp*Rh(bpy)(H2O]2+促进的P450催化：细胞色素C的直接再生
Towards [Cp*Rh(bpy)(H2O]2+-promoted P450 catalysis: direct regeneration of CytC.
作者信息
Hollmann Frank, Schmid Andreas
机构信息
Institute of Biotechnology, Swiss Federal Institute of Technology, Zurich, Switzerland.
出版信息
J Inorg Biochem. 2009 Mar;103(3):313-5. doi: 10.1016/j.jinorgbio.2008.11.005. Epub 2008 Nov 20.
The organometallic complex pentamethylcyclopentadienyl rhodium 2,2'-bipyridin (Cp(*)Rh(bpy)(H(2)O)) was applied as regeneration catalyst for cytochrome C (CytC). Direct reduction of CytC-bound Fe(III) was achieved in this model system pointing towards a potential usefulness of this concept to promote cell-free P450 catalysis. In addition, controlled in situ provision with hydrogen peroxide was performed using Cp(*)Rh(bpy)(H(2)O) resulting in improved CytC-catalyzed sulfoxidation of thioanisol This work represents the first step towards the direct-Cp(*)Rh(bpy)(H(2)O) catalyzed regeneration of P450 monooxygenases and peroxidases.
有机金属配合物五甲基环戊二烯基铑2,2'-联吡啶（[Cp()Rh(bpy)(H₂O)]²⁺）被用作细胞色素C（CytC）的再生催化剂。在该模型系统中实现了对CytC结合的Fe(III)的直接还原，这表明该概念在促进无细胞P450催化方面具有潜在用途。此外，使用[Cp()Rh(bpy)(H₂O)]²⁺进行了过氧化氢的原位可控供应，从而改善了CytC催化的苯甲硫醚的硫氧化反应。这项工作是迈向直接由[Cp(*)Rh(bpy)(H₂O)]²⁺催化P450单加氧酶和过氧化物酶再生的第一步。
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