Haschke-Becher Elisabeth, Brunser Oscar, Cruchet Sylvia, Gotteland Martin, Haschke Ferdinand, Bachmann Claude
Central Laboratory, University Hospital of Neurology, Paracelsus Medical University, Salzburg, Austria.
Ann Nutr Metab. 2008;53(3-4):240-4. doi: 10.1159/000185642. Epub 2008 Dec 16.
BACKGROUND/AIMS: Supplementation with certain probiotics can improve gut microbial flora and immune function but should not have adverse effects. This study aimed to assess the risk of D-lactate accumulation and subsequent metabolic acidosis in infants fed on formula containing Lactobacillus johnsonii (La1).
In the framework of a double-blind, randomized controlled trial enrolling 71 infants aged 4-5 months, morning urine samples were collected before and 4 weeks after being fed formulas with or without La1 (1 x 10(8)/g powder) or being breastfed. Urinary D- and L-lactate concentrations were assayed by enzymatic, fluorimetric methods and excretion was normalized per mol creatinine.
At baseline, no significant differences in urinary D-/L-lactate excretion among the formula-fed and breastfed groups were found. After 4 weeks, D-lactate excretion did not differ between the two formula groups, but was higher in both formula groups than in breastfed infants. In all infants receiving La1, urinary D-lactate concentrations remained within the concentration ranges of age-matched healthy infants which had been determined in an earlier study using the same analytical method. Urinary L-lactate also did not vary over time or among groups.
Supplementation of La1 to formula did not affect urinary lactate excretion and there is no evidence of an increased risk of lactic acidosis.
背景/目的：补充某些益生菌可改善肠道微生物群和免疫功能，但不应产生不良影响。本研究旨在评估喂食含约氏乳杆菌（La1）配方奶的婴儿中D-乳酸积累及随后发生代谢性酸中毒的风险。
在一项纳入71名4-5个月大婴儿的双盲随机对照试验框架内，在喂食含或不含La1（1×10⁸/g粉末）配方奶或母乳喂养前及4周后收集晨尿样本。采用酶促荧光法测定尿中D-和L-乳酸浓度，并以每摩尔肌酐排泄量进行标准化。
基线时，配方奶喂养组和母乳喂养组之间尿D-/L-乳酸排泄无显著差异。4周后，两个配方奶组的D-乳酸排泄无差异，但两个配方奶组均高于母乳喂养婴儿。在所有接受La1的婴儿中，尿D-乳酸浓度保持在先前使用相同分析方法确定的年龄匹配健康婴儿的浓度范围内。尿L-乳酸也未随时间或组间变化。
在配方奶中添加La1不影响尿乳酸排泄，且无证据表明乳酸酸中毒风险增加。
