Lacerda-Pinheiro Sally, Marchadier Arnaud, Donãs Patricio, Septier Dominique, Benhamou Laurent, Kellermann Odile, Goldberg Michel, Poliard Anne
Laboratoire de Différenciation Cellulaire, Cellules Souches et Prions, IFR- 2937 CNRS, Villejuif, France.
Open Dent J. 2008;2:67-72. doi: 10.2174/1874210600802010067. Epub 2008 Apr 29.
The continuously growing rodent incisor is a widely used model to investigate odontogenesis and mineralized tissue formation. This study focused on evaluating the mouse mandibular incisor as an experimental biological tool for analyzing in vivo the capacity of odontoblast-like progenitors or bioactive molecules to contribute to reparative dentinogenesis. We describe here a surgical procedure allowing direct access to the forming part of the incisor dental pulp Amelogenin peptide A+4 adsorbed on agarose beads, or dental pulp progenitor cells were implanted in the pulp following this procedure. After 10 days A+4 induced the formation of an osteodentin occluding almost the totality of the pulp compartment. Implantation of progenitor cells leads to formation of islets of osteodentin-like structures located centrally in the pulp. These pilot studies validate the incisor as an experimental model to test the capacity of progenitor cells or bioactive molecules to induce the formation of reparative dentin.
持续生长的啮齿动物门牙是研究牙发生和矿化组织形成的广泛使用的模型。本研究着重评估小鼠下颌门牙作为一种实验生物学工具，用于在体内分析成牙本质细胞样祖细胞或生物活性分子促成修复性牙本质形成的能力。我们在此描述一种外科手术方法，该方法可直接进入门牙牙髓的形成部分，将吸附在琼脂糖珠上的釉原蛋白肽A+4或牙髓祖细胞按照此方法植入牙髓。10天后，A+4诱导形成了几乎封闭整个牙髓腔的骨样牙本质。祖细胞的植入导致牙髓中央形成骨样牙本质样结构的小岛。这些初步研究证实门牙作为一种实验模型可用于测试祖细胞或生物活性分子诱导修复性牙本质形成的能力。
