Estrela Carlos, Estrela Cyntia Rodrigues de Araújo, Hollanda Augusto César Braz, Decurcio Daniel de Almeida, Pécora Jesus Djalma
Department of Oral Science, Federal University of Goiás, Goiânia, GO, Brazil.
J Appl Oral Sci. 2006 Jan;14(1):33-7. doi: 10.1590/s1678-77572006000100007.
The purpose of this research was to verify the influence of Iodoform on antimicrobial potential of calcium hydroxide. S. aureus, E. faecalis, P. aeruginosa, B. subtilis, C. albicans were the biological indicators. The substances tested were: calcium hydroxide + saline; calcium hydroxide + Iodoform + saline; Iodoform + saline. For the agar diffusion test, 18 Petri plates with 20 ml of BHI agar were inoculated with the microbial suspensions. Fifty-four cavities were made and filled with the substances tested. The diameters of microbial inhibition were then measured. In direct exposure test, 162 #50 sterile absorbent paper points were immersed in the experimental suspensions for 5 min, and covered with the pastes. At intervals of 24, 48 and 72 hours, the paper points were immersed in 10 ml of Letheen Broth, followed by incubation at 37 degrees C for 48h. Microbial growth was evaluated by turbidity of the culture medium. A 0.1 ml inoculum obtained from the Letheen Broth was transferred to 7 ml of BHI, and incubated at 37 degrees C for 48h. Bacterial growth was again evaluated by turbidity of the culture medium. The calcium hydroxide associated with the saline or the iodoform plus saline showed antimicrobial effectiveness in both experimental methods. The iodoform paste presented antimicrobial ineffectiveness for the agar diffusion test on all biological microorganisms and for the direct exposure test on B. subtilis and on the mixture.
本研究的目的是验证碘仿对氢氧化钙抗菌潜力的影响。金黄色葡萄球菌、粪肠球菌、铜绿假单胞菌、枯草芽孢杆菌、白色念珠菌为生物指标菌。所测试的物质有：氢氧化钙+生理盐水；氢氧化钙+碘仿+生理盐水；碘仿+生理盐水。对于琼脂扩散试验，向18个装有20 ml脑心浸液琼脂的培养皿中接种微生物悬液。制作54个孔并填充所测试的物质。然后测量微生物抑制直径。在直接接触试验中，将162个#50无菌吸水纸尖浸入实验悬液中5分钟，并用糊剂覆盖。在24、48和72小时的间隔时间，将纸尖浸入10 ml Letheen肉汤中，然后在37℃下孵育48小时。通过培养基的浊度评估微生物生长。从Letheen肉汤中获得的0.1 ml接种物转移至7 ml脑心浸液中，并在37℃下孵育48小时。再次通过培养基的浊度评估细菌生长。与生理盐水或碘仿加生理盐水相关的氢氧化钙在两种实验方法中均显示出抗菌效果。碘仿糊剂在所有生物微生物的琼脂扩散试验以及对枯草芽孢杆菌和混合物的直接接触试验中均表现出抗菌无效性。
