Tanomaru Filho Mário, Yamashita José Carlos, Leonardo Mario Roberto, da Silva Léa Assed Bezerra, Tanomaru Juliane Maria Guerreiro, Ito Izabel Yoko
Department of Endodontics, Araraquara Dental School, University of the State of São Paulo, UNESP, Araraquara, SP, Brazil.
J Appl Oral Sci. 2006 Apr;14(2):105-10. doi: 10.1590/s1678-77572006000200008.
The aim of this study was to evaluate the antimicrobial effect of biomechanical preparation using different irrigating solutions. Seventy-eight root canals from premolars of four dogs were used. After experimental induction of periapical lesions, the root canals were prepared using the following solutions for irrigation: Group 1) 2.5% sodium hypochlorite (NaOCl); Group 2) 2% chlorhexidine (CHX); Group 3) saline solution and Group 4) control group with no biomechanical preparation. The microbiological evaluation of the root canals was performed by counting the colony forming units (CFUs) using different culture mediums. Two absorbent paper cones were used in each root canal in order to collect the microbiological samples before, and thirty days after the biomechanical preparation. The culture plates were incubated in aerobic, anaerobic and microaerophilic environment. Statistical evaluation was carried out using analysis of variance, Tukey and Student tests. The results demonstrated that there was reduction in the number of microorganisms in the NaOCl and CHX groups (p<0.05). There was greater effectiveness in the chlorhexidine group. The group that used saline solution and the control group presented an increased number of microorganisms. It can be concluded that the use of antimicrobial irrigating solutions during biomechanical preparation promotes the reduction of endodontic microbiota. However, a considerable number of microorganisms were still observed.
本研究的目的是评估使用不同冲洗液进行生物力学预备的抗菌效果。使用了来自四只狗的前磨牙的78个根管。在实验性诱导根尖周病变后，使用以下溶液进行根管冲洗：第1组）2.5%次氯酸钠（NaOCl）；第2组）2%氯己定（CHX）；第3组）生理盐水溶液；第4组）未进行生物力学预备的对照组。通过使用不同培养基计数菌落形成单位（CFU）对根管进行微生物学评估。在每个根管中使用两个吸水纸尖，以便在生物力学预备前和预备后30天收集微生物样本。培养平板在需氧、厌氧和微需氧环境中孵育。使用方差分析、Tukey检验和Student检验进行统计学评估。结果表明，NaOCl组和CHX组中的微生物数量减少（p<0.05）。氯己定组的效果更佳。使用生理盐水溶液的组和对照组中的微生物数量增加。可以得出结论，在生物力学预备期间使用抗菌冲洗液可促进牙髓微生物群的减少。然而，仍观察到相当数量的微生物。
