Bhasin Prashant, Sharma Mohit, Bindal Deepti, Tomar Deepak, Sarin Anurag, Sharma Naren
Department of Conservative Dentistry and Endodontics, Shree Bankey Bihari Dental College and Research Centre, Ghaziabad, Uttar Pradesh, India.
Department of Conservative Dentistry and Endodontics, Shree Bankey Bihari Dental College and Research Centre, Ghaziabad, Uttar Pradesh, India, Phone: +919953523848, e-mail:
J Contemp Dent Pract. 2019 Feb 1;20(2):221-225.
The present study was conducted to evaluate and compare antimicrobial effect of 5.25% sodium hypochlorite (NaOCl), 2% (CHX) and (NAC) irrigating solutions against () and ().
The present study was conducted on 40 freshly extracted noncarious permanent mandibular incisors teeth of both genders (males-12, females-14). In all teeth, root canal preparation was done up to size 40 K-file. Roots were sterilized and microbial suspension of mixed culture of the tested microorganisms was inoculated into canals and incubated for 48 h. Teeth were divided into four groups, group I (5.25% sodium hypochlorite), group II (2% chlorhexidine), group III (200 mg/mL NAC) and group IV (sterile distilled water). The antimicrobial effect in each group was compared.
Statistical evaluation was completed using statistical software Statistical Package for the Social Sciences (SPSS). Planktonic bacterial count was lowest in group III followed by group I, group II and group IV. count was 6.14 ± 0.12 in group I, 5.76 ± 0.44 in group II, 3.88 ± 0.08 in group III and 11.98 ± 1.04 in group IV. The difference was significant ( < 0.05). The proportion of dead cell found to be 0.04± 0.01, 0.72 ± 0.06, 0.01 ± 0.06 and 1.02 ± 0.11 in groups I, II, III and IV respectively. The difference was significant ( < 0.05).
NAC proved to be effective against and . This solution can be considered alternative in root canal infections in addition with CHX and NaOCl.
Effectiveness of three different irrigating solutions was compared and NAC found to be more efficient in decreasing bacterial count. Hence, NAC can be precisely used in irrigating root canals to achieve optimal clinical outcomes particularly regarding reoccurrences of infections. Furthermore, NAC could be proved as a promising innovation in future endodontic methodologies.
本研究旨在评估和比较5.25%次氯酸钠（NaOCl）、2%氯己定（CHX）和200mg/mL N-乙酰半胱氨酸（NAC）冲洗液对粪肠球菌（E. faecalis）和金黄色葡萄球菌（S. aureus）的抗菌效果。
本研究选取40颗新鲜拔除的无龋永久性下颌切牙，男女不限（男性12颗，女性14颗）。所有牙齿均用40号K锉进行根管预备。对牙根进行消毒，将测试微生物的混合培养物的微生物悬液接种到根管中并孵育48小时。牙齿分为四组，第一组（5.25%次氯酸钠），第二组（2%氯己定），第三组（200mg/mL NAC）和第四组（无菌蒸馏水）。比较每组的抗菌效果。
使用社会科学统计软件包（SPSS）完成统计评估。浮游细菌计数在第三组中最低，其次是第一组、第二组和第四组。第一组的计数为6.14±0.12，第二组为5.76±0.44，第三组为3.88±0.08，第四组为11.98±1.04。差异具有统计学意义（P<0.05）。在第一组、第二组、第三组和第四组中发现的死细胞比例分别为0.04±0.01、0.72±0.06、0.01±0.06和1.02±0.11。差异具有统计学意义（P<0.05）。
NAC被证明对粪肠球菌和金黄色葡萄球菌有效。该溶液可被视为根管感染中除CHX和NaOCl之外的替代方案。
比较了三种不同冲洗液的有效性，发现NAC在降低细菌计数方面更有效。因此，NAC可精确用于冲洗根管以实现最佳临床效果，特别是在感染复发方面。此外，NAC可能被证明是未来牙髓治疗方法中的一项有前途的创新。
