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[Molecular biological study of the vaccinia virus genome. IV. The late nonstructural 36K protein of vaccinia virus is vitally important].

作者信息

Shchelkunov S N, Riazankina O I, Gashnikov P B, Totmenin A B, Chizhikov V E, Malygin E G

出版信息

Mol Biol (Mosk). 1991 Mar-Apr;25(2):396-404.

PMID:1908941
Abstract

Two genetics markers: the herpes simplex virus thymidine kinase and Escherichia coli beta-galactosidase genes were inserted into the 36K protein gene of vaccinia virus located in a HindIII-P DNA fragment. An unstability of recombinant viruses with Lac(+)-phenotype were discovered. A mechanism of viruses unstable variants formation was proposed, it was confirmed by the results of hybridisation analyses of virus recombinant genomes. The importance of a late nonstructural 36K protein gene for virus reproduction was demonstrated.

摘要

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