Queiroz D A, Cortelli J R, Holzhausen M, Rodrigues E, Aquino D R, Saad W A
Department of Dentistry, University of Taubaté, São Paulo, Brazil.
Clin Oral Investig. 2009 Sep;13(3):263-7. doi: 10.1007/s00784-008-0238-0. Epub 2008 Dec 16.
It is believed that an increased arginase activity may lead to less nitric oxide production, which consequently increases the susceptibility to bacterial infection. Considering the hypothesis that smoking may alter the arginase activity and that smoking is considered a risk factor to dental implant survival, the present study aimed at evaluating the effect of smoking on the salivary arginase activity of patients with dental implants. Salivary samples of 41 subjects were collected: ten non-smoking and with no dental implants (group A), ten non-smoking subjects with dental implants (group B), ten smoking subjects with implants (group C), and 11 smoking subjects with no dental implants (group D). The levels of salivary arginase activity were determined by the measurement of L-ornithine and expressed as mIU/mg of protein. A significant increase in the salivary arginase activity was verified in groups C (64.26 +/- 16.95) and D (49.55 +/- 10.01) compared to groups A (10.04 +/- 1.95, p = 0.00001 and p = 0.0110, groups C and D, respectively) and B (11.77 +/- 1.45, p = 0.00001 and p = 0.0147, groups C and D, respectively). No significant difference was found between groups C and D (p = 0.32). Within the limits of the present study, it can be concluded that salivary arginase activity is increased in smoking subjects with dental implants in contrast to non-smoking subjects with dental implants, therefore suggesting a possible mechanism by which cigarette smoking may lead to implant failure. The analysis of salivary arginase activity may represent an important tool to prevent implant failure in the near future.
据信，精氨酸酶活性增加可能导致一氧化氮生成减少，从而增加对细菌感染的易感性。考虑到吸烟可能改变精氨酸酶活性这一假说，且吸烟被认为是牙种植体存留的一个风险因素，本研究旨在评估吸烟对牙种植体患者唾液精氨酸酶活性的影响。收集了41名受试者的唾液样本：10名不吸烟且无牙种植体者（A组），10名不吸烟的牙种植体受试者（B组），10名吸烟的牙种植体受试者（C组），以及11名吸烟且无牙种植体者（D组）。通过测量L-鸟氨酸来确定唾液精氨酸酶活性水平，并以每毫克蛋白质的毫国际单位表示。与A组（10.04±1.95，C组和D组分别为p = 0.00001和p = 0.0110）和B组（11.77±1.45，C组和D组分别为p = 0.00001和p = 0.0147）相比，C组（64.26±16.95）和D组（49.55±10.01）的唾液精氨酸酶活性显著增加。C组和D组之间未发现显著差异（p = 0.32）。在本研究的范围内，可以得出结论，与不吸烟的牙种植体受试者相比，吸烟的牙种植体受试者唾液精氨酸酶活性增加，因此提示吸烟可能导致种植体失败的一种可能机制。唾液精氨酸酶活性分析可能成为在不久的将来预防种植体失败的一项重要工具。
