Dewi Rizna T, Iskandar Yetty M, Hanafi M, Kardono L B S, Angelina Marisa, Dewijanti Indah D, Banjarnahor Sofna D S
Research Centre for Chemistry, Indonesian Institute of Science Kawasan Puspiptek, Serpong 15310, Indonesia.
Pak J Biol Sci. 2007 Sep 15;10(18):3131-5. doi: 10.3923/pjbs.2007.3131.3135.
The compounds that could inhibit the activity of a-glucosidase are potentially used for antidiabetic by suppressing postprandial hyperglycemia. This research aimed to investigate the hypoglycemic activity in A. terreus koji extracted by ethyl acetate. The extracts was dissolved in methanol: water (1:4), followed by fractionations with n-hexane, methylene chloride and ethyl acetate. Each fraction was assayed for its activity against a-glucosidase. The active fraction was purified by column chromatography using silica gel and resin as adsorbent. The kopi extract showed potential as alpha-glucosidase inhibition with IC50 <10 microg mL(-1) and showed combination of non-competitive and uncompetitive inhibition mode against a-glucosidase. Ethyl acetate fraction showed potential as inhibitor alpha-glucosidase with IC50 = 8.6 microg mL(-1). In animal experiment, active fraction (F10-4) of ethyl acetate fraction suppressed the increase of postprandial blood glucosidase level compare to the control. Thus it showed potential as alpha-glucosidase inhibitor and demonstrated depressed postprandial blood glucose level and may have potential use in the management of type 2 diabetes.
能够抑制α-葡萄糖苷酶活性的化合物可通过抑制餐后高血糖而潜在地用于抗糖尿病。本研究旨在调查用乙酸乙酯提取的土曲霉酒曲中的降血糖活性。提取物溶解于甲醇：水（1:4）中，然后依次用正己烷、二氯甲烷和乙酸乙酯进行分级分离。对每个级分进行α-葡萄糖苷酶活性测定。活性级分通过使用硅胶和树脂作为吸附剂的柱色谱法进行纯化。kopi提取物显示出α-葡萄糖苷酶抑制潜力，IC50 <10 μg mL(-1)，并且对α-葡萄糖苷酶表现出非竞争性和非竞争性抑制模式的组合。乙酸乙酯级分显示出作为α-葡萄糖苷酶抑制剂的潜力，IC50 = 8.6 μg mL(-1)。在动物实验中，乙酸乙酯级分的活性级分（F10-4）与对照组相比抑制了餐后血糖水平的升高。因此，它显示出作为α-葡萄糖苷酶抑制剂的潜力，并证明可降低餐后血糖水平，可能在2型糖尿病的管理中有潜在用途。
