利用RAPD标记对部分油橄榄（Olea europaea L.）品种进行遗传多样性研究。

Study of genetic diversity in some olive (Olea europaea L.) cultivars by using RAPD markers.

作者信息

Sheidai M, Vaezi-Joze S, Shahriari Z H, Noormohammadi Z, Parsian H, Farahanei F

机构信息

Department of Biology, Shahid Beheshti University, Tehran, Iran.

出版信息

Pak J Biol Sci. 2007 Sep 1;10(17):2972-5. doi: 10.3923/pjbs.2007.2972.2975.

DOI:10.3923/pjbs.2007.2972.2975

PMID:19090211

Abstract

RAPD markers variations were studied in eleven olive cultivars. Seventeen RAPD primers out of 27 produced 610 bands in total. Four hundred forty four bands were polymorphic bands (72.70 %) and 166 bands were monomorphic (27.20 %). Primer C05 produced the highest number of bands (56), while primer C03 produced the lowest number of bands (7). Specific bands were observed in some of the cultivars, which may be used in the cultivars discrimination. Different similarity coefficients determined among the cultivars studied, showed the highest value of similarity between cultivars Kroneiki and Manzanila. Different clustering methods showed distinctness of the olive cultivars studied. The grouping of the cultivars did not correlate completely with their place of origin or fruit characteristics.

摘要

对11个橄榄品种的随机扩增多态性DNA（RAPD）标记变异进行了研究。27个RAPD引物中的17个共产生了610条带。444条带为多态性条带（72.70%），166条带为单态性条带（27.20%）。引物C05产生的条带数量最多（56条），而引物C03产生的条带数量最少（7条）。在一些品种中观察到了特异性条带，可用于品种鉴别。在所研究的品种之间确定的不同相似系数显示，品种Kroneiki和Manzanila之间的相似性值最高。不同的聚类方法表明所研究的橄榄品种具有明显差异。品种的分组与它们的起源地或果实特征并不完全相关。