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土耳其本土油橄榄(油橄榄属欧洲油橄榄种)品种间的遗传关系

Genetic Relationships Among Olive (Olea europaea L.) Cultivars Native to Turkey.

作者信息

Sakar Ebru, Unver Hulya, Bakir Melike, Ulas Mehmet, Sakar Zeynep Mujde

机构信息

Department of Horticulture, Faculty of Agriculture, Harran University, Sanliurfa, Turkey.

Ankara University Kalecik Vocational School, Kalecik, Ankara, Turkey.

出版信息

Biochem Genet. 2016 Aug;54(4):348-359. doi: 10.1007/s10528-016-9723-3. Epub 2016 Feb 22.

Abstract

Olive is a widely cultivated, mainly in the Mediterranean region, and economically important fruit species used as both olive oil and table olive consumption. In Turkey, more than 50 olive cultivars have been authorized for commercial plantations, representing the developmental base for the olive industry. The aim of the present study was to identify genetic relationships among the most widely grown 27 olive cultivars in Turkey, using microsatellite or simple sequence repeat markers. Nine well-known foreign olive cultivars from different countries are also included in the study to compare the Turkish cultivars. To determine genetic relationship and diversity, 10 SSR loci (DCA3, DCA9, DCA15, DCA18, UDO4, UDO9, UDO11, UDO12, UDO24, UDO28) were used. Jaccard's similarity coefficient and the UPGMA method for cluster analysis were performed using the software NTSYSpc. The results showed that the number of alleles per locus ranging from 4 (UDO4, UDO9, UDO11, UDO12, DCA15) to 12 (DCA9) presenting high polymorphism. There were no identical cultivars. High similarity was shown by cultivars Maviand Adana topağı (0.754). The most genetically divergent cultivars, Domat-Meski (0.240) and Domat-NizipYağlık (0.245), were also identified.

摘要

油橄榄是一种广泛种植的水果品种,主要种植在地中海地区,在经济上具有重要意义,其果实既用于生产橄榄油,也用于制作食用橄榄。在土耳其,已有50多个油橄榄品种被授权用于商业种植园,这是油橄榄产业发展的基础。本研究的目的是利用微卫星或简单序列重复标记,确定土耳其种植最广泛的27个油橄榄品种之间的遗传关系。该研究还纳入了9个来自不同国家的著名外国油橄榄品种,以与土耳其品种进行比较。为了确定遗传关系和多样性,使用了10个SSR位点(DCA3、DCA9、DCA15、DCA18、UDO4、UDO9、UDO11、UDO12、UDO24、UDO28)。使用软件NTSYSpc进行Jaccard相似系数计算和聚类分析的UPGMA方法。结果表明,每个位点的等位基因数量从4个(UDO4、UDO9、UDO11、UDO12、DCA15)到12个(DCA9)不等,呈现出高度多态性。没有相同的品种。Mavi和阿达纳托帕ğı品种表现出高度相似性(0.754)。还确定了遗传差异最大的品种,多马特-梅斯基(0.240)和多马特-尼济皮亚克利克(0.245)。

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