Aniagyei Stella E, Kennedy Chelsea J, Stein Barry, Willits Deborah A, Douglas Trevor, Young Mark J, De Mrinmoy, Rotello Vincent M, Srisathiyanarayanan D, Kao C Cheng, Dragnea Bogdan
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA.
Nano Lett. 2009 Jan;9(1):393-8. doi: 10.1021/nl8032476.
A study of the in vitro nanoparticle-templated assembly of a mutant of cowpea chlorotic mottle virus lacking most of the N-terminal domain (residues 4-37), NDelta34, is presented. Mutant empty proteins assemble into empty capsids with a much broader distribution of sizes than the wild-type virus. This increased flexibility in the assembly outcomes is known to be detrimental for the assembly process in the presence of molecular polyanions. However, when rigid polyanionic cores are used, such as nanoparticles, the assembly process is restored and virus-like particles form. Moreover, the breadth of the nanoparticle-templated capsid size distribution becomes comparable with the wild-type virus size distribution.
本文展示了一项关于豇豆花叶病毒（CCMV）一种突变体（缺失大部分N端结构域，即4 - 37位残基，NDelta34）的体外纳米颗粒模板组装研究。与野生型病毒相比，突变体空蛋白组装成的空衣壳尺寸分布范围更广。已知在存在分子聚阴离子的情况下，组装结果中这种增加的灵活性对组装过程不利。然而，当使用刚性聚阴离子核心，如纳米颗粒时，组装过程得以恢复并形成病毒样颗粒。此外，纳米颗粒模板衣壳尺寸分布的宽度变得与野生型病毒尺寸分布相当。
