Oya Kei, Tanaka Yuta, Saito Haruka, Kurashima Kazuya, Nogi Kazuya, Tsutsumi Harumi, Tsutsumi Yusuke, Doi Hisashi, Nomura Naoyuki, Hanawa Takao
Department of Metals, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Biomaterials. 2009 Mar;30(7):1281-6. doi: 10.1016/j.biomaterials.2008.11.030. Epub 2008 Dec 16.
The effect of a cell-adhesive peptide containing Arg-Gly-Asp (RGD) immobilized through poly(ethylene glycol) (PEG) on titanium (Ti) on calcification by MC3T3-E1 cells was investigated to develop a new surface modification technique using biofunctional molecules. RGD was immobilized on Ti through PEG, both terminals of which were terminated with -NH(2) and -COOH to combine with the Ti surface and RGD. PEG was immobilized on Ti with electrodeposition, and RGD, with immersion. For comparison, glycine was employed because it is the simplest molecule containing both -NH(2) and -COOH at its terminals. MC3T3-E1 cells were cultured and differentiation-induced on each specimen, and the cell calcification properties were investigated. As a result, there was no significant difference in the morphology and extension of MC3T3-E1 cells cultured on each specimen, while the number of cells cultured on RGD/PEG/Ti was the largest. After differentiation-induction, there was no significant difference in the ALP activity among all specimens. On the other hand, the level of cell calcification on RGD/PEG/Ti was the highest. Therefore, the hard tissue compatibility of Ti is improved by immobilizing RGD through functional molecules which have a long molecular chain.
研究了通过聚乙二醇（PEG）固定在钛（Ti）上的含精氨酸-甘氨酸-天冬氨酸（RGD）的细胞粘附肽对MC3T3-E1细胞钙化的影响，以开发一种使用生物功能分子的新型表面改性技术。RGD通过PEG固定在Ti上，PEG的两端分别用-NH(2)和-COOH封端，以便与Ti表面和RGD结合。PEG通过电沉积固定在Ti上，RGD通过浸泡固定。为了进行比较，使用了甘氨酸，因为它是末端同时含有-NH(2)和-COOH的最简单分子。在每个样本上培养并诱导MC3T3-E1细胞分化，并研究细胞钙化特性。结果，在每个样本上培养的MC3T3-E1细胞的形态和伸展没有显著差异，而在RGD/PEG/Ti上培养的细胞数量最多。诱导分化后，所有样本之间的碱性磷酸酶活性没有显著差异。另一方面，RGD/PEG/Ti上的细胞钙化水平最高。因此，通过具有长分子链的功能分子固定RGD可改善Ti的硬组织相容性。
