Yu Meng-Liu, Yu Meng-Fei, Zhu Li-Qin, Wang Tian-Tian, Zhou Yi, Wang Hui-Ming
The Affiliated Hospital of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China.
Biomed Res Int. 2015;2015:582359. doi: 10.1155/2015/582359. Epub 2015 Aug 31.
Cell sheet technology is a new strategy in tissue engineering which could be possible to implant into the body without a scaffold. In order to get an integrated cell sheet, a light-induced method via UV365 is used for cell sheet detachment from culture dishes. In this study, we investigated the possibility of cell detachment and growth efficiency on TiO2 nanodot films with RGD immobilization on light-induced cell sheet technology. Mouse calvaria-derived, preosteoblastic (MC3T3-E1) cells were cultured on TiO2 nanodot films with (TR) or without (TN) RGD immobilization. After cells were cultured with or without 5.5 mW/cm(2) UV365 illumination, cell morphology, cell viability, osteogenesis related RNA and protein expression, and cell detachment ability were compared, respectively. Light-induced cell detachment was possible when cells were cultured on TR samples. Also, cells cultured on TR samples showed better cell viability, alongside higher protein and RNA expression than on TN samples. This study provides a new biomaterial for light-induced cell/cell sheet harvesting.
细胞片技术是组织工程中的一种新策略，有望在无支架的情况下植入体内。为了获得完整的细胞片，采用通过UV365的光诱导方法从培养皿中分离细胞片。在本研究中，我们研究了在光诱导细胞片技术中，具有RGD固定化的TiO2纳米点膜上细胞分离和生长效率的可能性。将小鼠颅骨来源的前成骨细胞（MC3T3-E1）培养在具有（TR）或不具有（TN）RGD固定化的TiO2纳米点膜上。在用或不用5.5 mW/cm(2) UV365光照培养细胞后，分别比较细胞形态、细胞活力、成骨相关RNA和蛋白质表达以及细胞分离能力。当细胞在TR样品上培养时，光诱导细胞分离是可行的。此外，与TN样品相比，在TR样品上培养的细胞显示出更好的细胞活力，以及更高的蛋白质和RNA表达。本研究为光诱导细胞/细胞片收获提供了一种新的生物材料。
