抗脑膜炎奈瑟菌A、B和C群荚膜多糖多克隆抗体和单克隆抗体的制备及乳胶试剂的制备
Production of polyclonal and monoclonal antibodies against group A, B, and C capsular polysaccharides of Neisseria meningitidis and preparation of latex reagents.
作者信息
Nato F, Mazie J C, Fournier J M, Slizewicz B, Sagot N, Guibourdenche M, Postic D, Riou J Y
机构信息
Hybridolab, Institut Pasteur, Paris, France.
出版信息
J Clin Microbiol. 1991 Jul;29(7):1447-52. doi: 10.1128/jcm.29.7.1447-1452.1991.
Abstract
Polyclonal and monoclonal antibodies against capsular polysaccharides of Neisseria meningitidis serogroups A, B, and C were produced in order to develop immunological reagents allowing both the detection of soluble antigens during meningococcal meningitis and antigenic serogrouping of N. meningitidis cultures. The performance characteristics of monoclonal and polyclonal antibody latex reagents were compared. For the detection of soluble polysaccharide antigen, polyclonal antibody latex reagent was selected for N. meningitidis A and C. The latex reagent prepared with polyclonal antibodies against N. meningitidis B could not detect capsular polysaccharide even at 1 mg/ml. The monoclonal antibody B latex reagent which detected 100 ng of polysaccharide per ml was therefore chosen. For the serogroup identification of N. meningitidis, the use of a confirmatory test results in an overall specificity of 100% with polyclonal or monoclonal antibody latex reagents.
摘要
制备了针对A、B和C群脑膜炎奈瑟菌荚膜多糖的多克隆抗体和单克隆抗体，以开发免疫试剂，用于在脑膜炎球菌性脑膜炎期间检测可溶性抗原以及对脑膜炎奈瑟菌培养物进行抗原血清分组。比较了单克隆和多克隆抗体乳胶试剂的性能特征。对于可溶性多糖抗原的检测，选择了针对A群和C群脑膜炎奈瑟菌的多克隆抗体乳胶试剂。用针对B群脑膜炎奈瑟菌的多克隆抗体制备的乳胶试剂即使在1 mg/ml时也无法检测到荚膜多糖。因此选择了每毫升能检测100 ng多糖的单克隆抗体B乳胶试剂。对于脑膜炎奈瑟菌的血清群鉴定，使用确证试验时，多克隆或单克隆抗体乳胶试剂的总体特异性均为100%。
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