三种商业产品与对流免疫电泳法在检测脑脊液中抗原的比较评估
Comparative evaluation of three commercial products and counterimmunoelectrophoresis for the detection of antigens in cerebrospinal fluid.
作者信息
Tilton R C, Dias F, Ryan R W
出版信息
J Clin Microbiol. 1984 Aug;20(2):231-4. doi: 10.1128/jcm.20.2.231-234.1984.
Abstract
Three commercial products and counterimmunoelectrophoresis were evaluated for their ability to detect microbial antigens of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in cerebrospinal fluid from 157 patients suspected of having meningitis. Thirty-four patients were diagnosed as having bacterial meningitis by culture, microscopy, or antigen detection. The overall results showed the following detection percentages; counterimmunoelectrophoresis, 76%; Phadebact CSF, 76%; Directigen, 82%, and Bactigen, 93%. The results with purified antigen revealed that latex agglutination was more sensitive than coagglutination, which in turn was more sensitive than counterimmunoelectrophoresis.
摘要
对三种商业产品和对流免疫电泳检测157例疑似脑膜炎患者脑脊液中脑膜炎奈瑟菌、流感嗜血杆菌和肺炎链球菌微生物抗原的能力进行了评估。34例患者通过培养、显微镜检查或抗原检测被诊断为细菌性脑膜炎。总体结果显示以下检测百分比：对流免疫电泳为76%；Phadebact脑脊液检测为76%；Directigen检测为82%，Bactigen检测为93%。纯化抗原的检测结果显示，乳胶凝集比协同凝集更敏感，而协同凝集又比对流免疫电泳更敏感。
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