体液中细菌抗原检测：快速抗原浓缩及减少非特异性反应的方法
Bacterial antigen detection in body fluids: methods for rapid antigen concentration and reduction of nonspecific reactions.
作者信息
Doskeland S O, Berdal B P
出版信息
J Clin Microbiol. 1980 Apr;11(4):380-4. doi: 10.1128/jcm.11.4.380-384.1980.
Abstract
We sought procedures which would allow a rapid concentration in high yield of bacterial antigens from tissue fluids of patients and which could be applied also to protein-rich fluids like serum. Ethanol precipitation at a subzero temperature with albumin added as an antigen coprecipitant made it possible to achieve a more than 20-fold concentration of antigen in 15 min and a 200-fold concentration in 45 min. Heat-stable antigens could be concentrated from protein-rich fluids (like serum) after the sample had been deproteinized by boiling. Such heating (100 degrees C, 3 min) also liberated bacterial polysaccharides from antibody complexes and elminated the nonspecific interference of serum in enzyme-linked immunosorbent assay.
摘要
我们寻求这样的方法，它能快速、高产地从患者的组织液中浓缩细菌抗原，并且也能应用于血清等富含蛋白质的液体。在零下温度下加入白蛋白作为抗原共沉淀剂进行乙醇沉淀，可在15分钟内使抗原浓度提高20倍以上，在45分钟内提高200倍。在样品通过煮沸进行脱蛋白后，可从富含蛋白质的液体（如血清）中浓缩热稳定抗原。这种加热（100摄氏度，3分钟）还能从抗体复合物中释放细菌多糖，并消除血清在酶联免疫吸附测定中的非特异性干扰。
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