NEIKER-Tecnalia, Animal Health Department, 48160 Derio, Bizkaia, Spain.
Vet Microbiol. 2010 Jul 29;144(1-2):240-5. doi: 10.1016/j.vetmic.2009.12.041. Epub 2010 Jan 11.
The Eurasian wild boar (Sus scrofa) is increasingly relevant as a host for several pathogenic mycobacteria. We aimed to characterize the first experimental Mycobacterium avium subsp. avium (MAA) infection in wild boar in order to describe the lesions and the immune response as compared to uninfected controls. Twelve 1-4-month-old wild boar piglets were housed in class III bio-containment facilities. Four concentrations of MAA suspension were used: 10, 10(2) and 10(4) mycobacteria (2 animals each, oropharyngeal route) and 2.5 x 10(6) mycobacteria (2 animals each by the oropharyngeal and nasal routes). No clinical signs were observed and pathology evidenced a low pathogenicity of this MAA strain for this particular host. Bacteriological and pathological evidence of successful infection after experimental inoculation was found for the group challenged with 2.5 x 10(6) mycobacteria. These four wild boar showed a positive IFN-gamma response to the avian PPD and the real-time RT-PCR data revealed that three genes, complement component C3, IFN-gamma and RANTES, were significantly down regulated in infected animals. These results were similar to those found in naturally and experimentally M. bovis-infected wild boar and may constitute biomarkers of mycobacterial infection in this species.
欧亚野猪（Sus scrofa）作为几种致病性分枝杆菌的宿主越来越重要。我们旨在描述首次在野猪中发生的实验性禽分枝杆菌亚种（MAA）感染，以与未感染对照相比描述病变和免疫反应。将 12 只 1-4 个月大的野猪仔猪饲养在三级生物安全设施中。使用了 4 种 MAA 悬浮液浓度：10、10（2）和 10（4）个分枝杆菌（每组 2 只，经口途径）和 2.5 x 10（6）个分枝杆菌（每组 2 只，经口和鼻途径）。未观察到临床症状，病理学证据表明该 MAA 株对该特定宿主的致病性较低。在接受 2.5 x 10（6）个分枝杆菌挑战的组中，发现了实验接种后成功感染的细菌学和病理学证据。这四只野猪对禽 PPD 表现出阳性 IFN-γ反应，实时 RT-PCR 数据显示，三种基因，补体成分 C3、IFN-γ和 RANTES，在感染动物中明显下调。这些结果与在自然和实验性牛分枝杆菌感染的野猪中发现的结果相似，可能是该物种分枝杆菌感染的生物标志物。
