Valdes-Stauber N, Götz H, Busse M
Department of Bacteriology, South German Experimental and Research Station in Dairying, Technical University of Munich, Freising, F.R.G.
Int J Food Microbiol. 1991 Jun;13(2):119-30. doi: 10.1016/0168-1605(91)90054-s.
A total of 187 coryneform bacteria were isolated from red smear and screened for inhibitory effects against 16 strains of Listeria species. Culture filtrates from Brevibacterium linens (16 strains), Arthrobacter nicotianae (4 strains) and Arthrobacter nucleogenes (3 strains) showed clear zones of inhibition. The antagonistic effect was seen against 26 to 87% of 91 Listeria strains tested. A. nicotianae and A. nucleogenes were more effective against Listeria innocua and Listeria ivanovii than against Listeria monocytogenes. No species specifically was observed for B. linens, but there was a difference regarding the inhibitory activity of individual culture filtrates. When culture filtrates of the test strains were added to Listeria broth cultures, the maximum growth level was not attained. Inhibition in broth cultures was dependent on the concentration of culture filtrates. Culture filtrates from the late stationary phase had a stronger inhibitory effect on the growth of L. monocytogenes. The nature of the inhibitory effects remained unclear. Attempts to characterize the nature of the antagonism showed that the culture filtrates lost their inhibitory activity upon heating, and the molecular size of the inhibitory substances were greater than 12-14 kDa.
从红色涂片中共分离出187株棒状杆菌，并筛选了它们对16株李斯特菌属菌株的抑制作用。来自表皮短杆菌（16株）、烟草节杆菌（4株）和产核苷节杆菌（3株）的培养滤液显示出明显的抑菌圈。在测试的91株李斯特菌中，对26%至87%的菌株观察到了拮抗作用。烟草节杆菌和产核苷节杆菌对无害李斯特菌和伊万诺夫李斯特菌的作用比对单核细胞增生李斯特菌更有效。表皮短杆菌未观察到特定的菌种差异，但个别培养滤液的抑制活性存在差异。当将测试菌株的培养滤液添加到李斯特菌肉汤培养物中时，未达到最大生长水平。肉汤培养物中的抑制作用取决于培养滤液的浓度。来自稳定期后期的培养滤液对单核细胞增生李斯特菌的生长具有更强的抑制作用。抑制作用的性质仍不清楚。对拮抗作用性质的表征尝试表明，培养滤液加热后失去抑制活性，抑制物质的分子大小大于12 - 14 kDa。
